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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 115,229 results for: CIL

Showing: 1101 - 1120 of 115229 results

  1. pcpBpaRS

    Plasmid
    #196484
    Purpose
    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (synthetase), Pol III tRNA promoter (tRNA)
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196484-plasmid-map-sequence-id-388864

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  5. Luc-noPS

    Plasmid
    #177940
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177940-plasmid-map-sequence-id-348112

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  7. pTK331

    Plasmid
    #59572
    Purpose
    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI
    Depositor
    Ron Weiss
    Article
    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038
    Insert
    SGP(16) 2xK-turn Kozak EGFP attB2 AscI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    59572-plasmid-map-sequence-id-92977

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  11. pcDNA3-ER-CaNAR2

    Plasmid
    #64732
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CYP450-CaNAR2
    Use
    Tags
    Cerulean3, N-terminal 27 amino acids from CYP450,…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64732-plasmid-map-sequence-id-109351

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  14. pcDNA3-PM-CaNAR2

    Plasmid
    #64730
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    Lyn-CaNAR2
    Use
    Tags
    Cerulean3, N-terminal targeting sequence from Lyn…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64730-plasmid-map-sequence-id-109348

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  15. pALOD4 non-binding mutant

    Plasmid
    #111027
    Purpose
    Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)
    Depositor
    Arun Radhakrishnan
    Article
    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d
    Insert
    Domain 4 of Anthrolysin O
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Mutations: S404C, C472A, G501A, T502A, T503A, L5…
    Promoter
    T7
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111027-plasmid-map-sequence-id-214042

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  19. pJH3449

    Plasmid
    #179576
    Purpose
    Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP
    Depositor
    Mei Zhen
    Article
    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1
    Insert
    tomm20::miniSOG (tomm-20 Nematode)
    Use
    Tags
    BFP
    Expression
    Worm
    Mutation
    Promoter
    Plgc-55B
    Available since
    March 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    179576-plasmid-map-sequence-id-355663

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Showing: 1101 - 1120 of 115229 results