We narrowed to 22,140 results for: his

1. pFRT/TO/HIS/FLAG/HA-PES1

   Plasmid

   #38085

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   PES1 pescadillo ribosomal biogenesis factor 1 (PES1 Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p2CT-His-MBP-Lbu_C2c2_R1079A

   Plasmid

   #83494

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R1079A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R1079A

   Promoter

   T7

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHub10-His-YFP-PIP2;1

   Plasmid

   #99386

   Purpose

   overexpression and isolation of recombinant At-PIP2;1 protein

   Depositor

   Ning Li

   Article

   Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

   Insert

   PIP2;1 (PIP2A Mustard Weed)
   

   Use

   Tags

   His and YFP

   Expression

   Plant

   Mutation

   Promoter

   2xCaMV35S

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFRT/TO/HIS/FLAG/HA-BTF3

   Plasmid

   #38075

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   BTF3 basic transcription factor 3 (BTF3 Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHybr_r2a>mG2a-srt-his

   Plasmid

   #124803

   Purpose

   HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.

   Depositor

   Martijn Verdoes, Ferenc Scheeren

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Murine IgG2a
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a(+) 6xHis-TEV-IFIT3

   Plasmid

   #53559

   Purpose

   bacterial expression of IFIT3

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT3 (IFIT3 Human)
   

   Use

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pENTR-HLA-B0702-His

   Plasmid

   #104849

   Purpose

   HLA-B0702 his tagged

   Depositor

   Sébastien Walchli

   Article

   Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

   Insert

   major histocompatibility complex, class I, B (HLA-B Human)
   

   Use

   Entry vector for gateway system

   Tags

   his

   Expression

   Mutation

   Promoter

   none

   Available Since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28b-LanCL2 (6xHis-LanCL2)

   Plasmid

   #73407

   Purpose

   Expresses His tagged LanCL2

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   LanCL2 (LANCL2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pFA6a-link-yoGFPgamma-SpHis5

   Plasmid

   #44859

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized GFPgamma with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoGFPgamma
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET51b-His-TEV-HaloTag7_dead_mt

    Plasmid

    #167267

    Purpose

    Overexpression of the dead variant (D106A) of HaloTag7 in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HaloTag7
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    D106A

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEB31 (hANGPTL8-V5-His lenti)

    Plasmid

    #109116

    Purpose

    V5 and His-tagged human ANGPTL8 in a lentiviral vector

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL8 (ANGPTL8 Human)
    

    Use

    Lentiviral

    Tags

    6x His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393

    Plasmid

    #216382

    Purpose

    Baculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Tetraspanin-12 (TSPAN12 Human)
    

    Use

    Baculovirus

    Tags

    6xHis-3C-splitGFP11

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBOB-CAG-fHis-H2A-Ub

    Plasmid

    #41797

    Depositor

    Inder Verma

    Article

    Zhu et al Nature. 2011 Sep 7;477(7363):179-84. doi

    Insert

    histone H2A
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA4/TO/myc-His-A-YAP2

    Plasmid

    #19060

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pAS1-rab-3P::his-24::CyOFP

    Plasmid

    #124347

    Purpose

    Nuclear Localized Pan-Neuronal CyOFP

    Depositor

    Andrew Leifer

    Insert

    rab-3, CyOFP (rab-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX4T1-GST-eIF4G1-His6

    Plasmid

    #37231

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    TIF4631 (TIF4631 Budding Yeast)
    

    Use

    Tags

    GST and His6

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pET21a-Med25-VBD-6His

    Plasmid

    #64773

    Purpose

    Bacterial expression of His-tagged Med25-VBD

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VBD (MED25 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    insert is 394-543 only

    Promoter

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pFA6a-link-yomEos2-SpHis5

    Plasmid

    #44849

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mEos2 with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomEos2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-DEST42-ARF6-V5-His6

    Plasmid

    #67415

    Purpose

    Expression of hArf6 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pET28a(+) 6xHis-TEV-IFIT2

    Plasmid

    #53558

    Purpose

    bacterial expression of IFIT2

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT2 (IFIT2 Human)
    

    Use

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

    Plasmid

    #79259

    Purpose

    Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

    Insert

    DmeIF4G (eIF4G1 Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    V5-His6 and lambdaN-HA

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA4-Myc-HisB-Sirt3

    Plasmid

    #24918

    Depositor

    Toren Finkel

    Article

    Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

    Insert

    Sirt3 (SIRT3 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pHIS3p:mRuby2-Tub1+3'UTR::TRP1

    Plasmid

    #50651

    Depositor

    Wei-Lih Lee

    Article

    Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

    Insert

    mRuby2-Tub1
    

    Use

    Yeast expression

    Tags

    mRuby2

    Expression

    Mutation

    Promoter

    HIS3p

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHIS3p:mTurquoise2-Tub1+3'UTR::TRP1

    Plasmid

    #50647

    Depositor

    Wei-Lih Lee

    Article

    Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

    Insert

    mTurquoise2-Tub1
    

    Use

    Yeast expression

    Tags

    mTurquoise2

    Expression

    Mutation

    Promoter

    HIS3p

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5/FRT/TO HIS HSPH3

    Plasmid

    #19536

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 4-like (HSPA4L Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA5/FRT/TO HIS HSPA2

    Plasmid

    #19539

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 2 (HSPA2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRPL28(K55M) SpHIS5 (pNTI666)

    Plasmid

    #115436

    Purpose

    Expresses K55M RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K55M; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFRT/TO/HIS/FLAG/HA-CSNK1E

    Plasmid

    #38078

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    CSNK1E casein kinase 1, epsilon (CSNK1E Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-deSpCas9-VP64-6xHis

    Plasmid

    #92117

    Purpose

    Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    T7

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. His-ZZ-TEV-BICDR1-GFP

    Plasmid

    #111657

    Purpose

    C-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1-GFP (Bicdl1 Mouse)
    

    Use

    Tags

    8xHis-tag, GFP, TEV site, and ZZ

    Expression

    Insect

    Mutation

    Optimised for Sf9 expression

    Promoter

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAS1-odr-4P::his-24::mNeptune

    Plasmid

    #124342

    Purpose

    Multicolor Worms for Neuronal ID

    Depositor

    Andrew Leifer

    Insert

    odr-4, mNeptune (odr-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA6-LRRC55-V5-Flag-His

    Plasmid

    #127819

    Purpose

    Expression of human LRRC55 in mammalian cells

    Depositor

    Jiusheng Yan

    Article

    Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

    Insert

    LRRC55 (LRRC55 Human)
    

    Use

    Tags

    V5, FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

    Plasmid

    #83484

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R1048A, H1053A

    Promoter

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET29b-dspB(E184Q)-6xHis

    Plasmid

    #175802

    Purpose

    Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFRT/TO/HIS/FLAG/HA-MYEF2

    Plasmid

    #38069

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    MYEF2 myelin expression factor 2 (MYEF2 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFastBac(His10-SNAP-Csk)

    Plasmid

    #177895

    Purpose

    Baculo expression of His10 tag fused to SNAPtag and human Csk

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Csk (CSK Human)
    

    Use

    Tags

    10xHis-TEV-TVMV-SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEU3-NII-GLICNot-C-His

    Plasmid

    #140187

    Purpose

    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, and NotI restriction site

    Depositor

    Tamás Mészáros

    Article

    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

    Type

    Empty backbone

    Use

    Expression vector for a cell-free system

    Tags

    6xHis and GST

    Expression

    Mutation

    Promoter

    T7

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5/FRT/TO HIS DNAJB6b

    Plasmid

    #19555

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB6b
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pC23-LIC-His-FLAG

    Plasmid

    #165477

    Purpose

    pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    TEV-FLAG-10x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

    Plasmid

    #204288

    Purpose

    yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    pHis3

    Available Since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge