We narrowed to 12,118 results for: pcDNA

1. pcDNA5/FRT/TO V5 DNAJB2b

   Plasmid

   #19524

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB2b (DNAJB2 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-sACE2(WT)-8his

   Plasmid

   #149268

   Purpose

   Mammalian expression plasmid for his-tagged sACE2

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   Short GSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA6-N-3XFLAG-Lrp6

   Plasmid

   #123595

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   low density lipoprotein receptor-related protein 6 (Lrp6 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 (-) hYAP 1-2alpha

   Plasmid

   #124143

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Megan Finch-Edmondson, Marius Sudol

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Mutation

   None (Wild Type)

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-3C-sGFP1-10_pcDNA3.1(-)

   Plasmid

   #182931

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Tags

   3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-YFP-PKD1

   Plasmid

   #31527

   Depositor

   Alexandra Newton

   Article

   Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

   Insert

   YFP-HA-Protein Kinase D (PRKD1 Human)
   

   Tags

   HA and YFP

   Expression

   Mammalian

   Available Since

   Nov. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1(+)GαoA-91-NLuc

   Plasmid

   #207151

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAO
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)G

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)GαoB-91-NLuc

   Plasmid

   #207152

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAO
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)G

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-MS2-HA-HsNBAS_AA

   Plasmid

   #148236

   Purpose

   Mammalian Expression of HsNBAS

   Depositor

   Elisa Izaurralde

   Insert

   HsNBAS (NBAS Human)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-eNOS-GFP

    Plasmid

    #22444

    Depositor

    William Sessa

    Article

    Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

    Insert

    WT eNOS-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.3-3XFLAG-AGO2

    Plasmid

    #136687

    Purpose

    Expresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)

    Depositor

    David Bartel

    Article

    McGeary et al Science. 2019 Dec 20;366(6472). pii: sci

    Insert

    argonaute RISC Catalytic Component 2 (AGO2 Human)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3-Flag-SPRTN-wt

    Plasmid

    #110214

    Purpose

    Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

    Depositor

    Ivana Marinovic-Terzic, Kristijan Ramadan

    Article

    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

    Insert

    SPRTN (SPRTN Human)
    

    Expression

    Mammalian

    Mutation

    P296L (see depositor comments below)

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-sACE2(WT)-sfGFP

    Plasmid

    #145171

    Purpose

    Mammalian expression plasmid for soluble ACE2-super folder GFP fusion

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 FLIPglu-600u

    Plasmid

    #13572

    Depositor

    Wolf Frommer

    Article

    Fehr et al J Biol Chem. 2003 May 23. 278(21):19127-

    Insert

    FLIPglu F16A
    

    Tags

    ECFP and EYFP

    Expression

    Mammalian

    Mutation

    F16A in binding protein

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3 MKK7b1(K149A)

    Plasmid

    #14674

    Depositor

    Roger Davis

    Article

    Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

    Insert

    MKK7b1(K149A) (Map2k7 Mouse)
    

    Expression

    Mammalian

    Mutation

    Lys-149Ala

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA-mito-GreenPy1Lowest

    Plasmid

    #239072

    Purpose

    Mammalian expression of mitochondria-targeted lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Lowest
    

    Expression

    Mammalian

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO GABARAP

    Plasmid

    #218427

    Purpose

    Expression of tagless GABARAP

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP (GABARAP Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-FLAG-Mo-Trip6

    Plasmid

    #27254

    Depositor

    Thomas Gilmore

    Article

    Wang et al Gene. 1999 Jul 8. 234(2):403-9.

    Insert

    Trip6 (Trip6 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA-FLAG-Hu-Trip6

    Plasmid

    #27255

    Depositor

    Thomas Gilmore

    Article

    Zhao et al Gene Expr. 1999 . 8(4):207-17.

    Insert

    Trip6 (TRIP6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pF152 pcDNA3.1(+)SOD1A4V

    Plasmid

    #26398

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Expression

    Mammalian

    Mutation

    A4V

    Available Since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pCDNA3 Flag BimL

    Plasmid

    #24233

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    Bim L (BCL2L11 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3-FLAG-Fos WT

    Plasmid

    #8966

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Oct. 4, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3.1(+)-OTI-TCRB_p2_TCRA

    Plasmid

    #131034

    Purpose

    Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRB (Tcrb Mouse)
    TCRA (Tcra Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV/T7

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3.1-CRISPR-Puro

    Plasmid

    #208646

    Purpose

    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

    Expression

    Mammalian

    Promoter

    CMV and U6 in tandem

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA-PANX1(HCVp)

    Plasmid

    #87698

    Purpose

    Express HCV protease-activatible pannexin-1 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PANX(HCVp)
    

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3 HA-humanTRAF3 (1098)

    Plasmid

    #66927

    Purpose

    Mammalian Expression of TRAF3

    Depositor

    Michael Karin

    Insert

    TRAF3 (TRAF3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA3.1-hRIPK1-Flag

    Plasmid

    #112487

    Purpose

    Mammalian expression of Flag-hRIPK1. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA3-RAB-AKARev

    Plasmid

    #118478

    Purpose

    ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-AKARev
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA6-N-3XFLAG-Dvl2

    Plasmid

    #123587

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    dishevelled segment polarity protein 2 (Dvl2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA3.1 (-) hYAP 1-2delta

    Plasmid

    #124146

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3/CyclinB1-GFP (28)

    Plasmid

    #39871

    Depositor

    Jonathon Pines

    Article

    Jackman et al Mol Biol Cell. 2002 Mar;13(3):1030-45.

    Insert

    CyclinB1 (CCNB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    V5A

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. RUNX1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176974

    Purpose

    Mammalian expression vector encoding RUNX1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    RUNX1 (RUNX1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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33. pcDNA3.1-MS2-HA-HshnRNPC1_Z

    Plasmid

    #148114

    Purpose

    Mammalian Expression of HshnRNPC1

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPC1 (HNRNPC Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3 Barr2 flag

    Plasmid

    #14685

    Depositor

    Robert Lefkowitz

    Article

    Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

    Insert

    beta-arrestin 2 (Arrb2 Rat)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

35. pCDNA3 Flag MKK4

    Plasmid

    #14615

    Depositor

    Roger Davis

    Article

    Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

    Insert

    MKK4 (MAP2K4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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36. hKCNQ3(WT).ires.mCherry-pcDNA3

    Plasmid

    #204362

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ3 (KCNQ3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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37. pcDNA3-10His-StayGold

    Plasmid

    #211362

    Purpose

    Mammalian expression of the bright and photostable StayGold fluorescent protein. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    StayGold
    

    Tags

    10xHis-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1 3xF-dyskerin

    Plasmid

    #126870

    Purpose

    Expresses 3xFlag dsykerin

    Depositor

    Kathleen Collins

    Article

    Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

    Insert

    Dyskerin
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-TopBP1-mCer

    Plasmid

    #211414

    Purpose

    mammalian expression of human TopBP1 C-terminally fused to mCerulean

    Depositor

    Helmut Pospiech

    Insert

    TopBP1 (TOPBP1 Human)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1-PDGFRA-V5/HIS

    Plasmid

    #234754

    Purpose

    Expression of human PDGFRA receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    PDGFRA receptor tyrosine kinase, full length, wildtype (PDGFRA Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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