Addgene

We narrowed to 12,997 results for: SHI

Showing: 2751 - 2800 of 12997 results

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pCCM935

Plasmid

#58202

Purpose

Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

unc-22 sgRNA

Use

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pMito.iGABASnFR.mRuby3

Plasmid

#112179

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-lexAp65

Plasmid

#138393

Purpose

RMCE vector for generating C-terminal T2A-lexA-p65 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-lexAp65

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRHDAd-TKVenus

Plasmid

#26422

Depositor

Kohnosuke Mitani

Article

Suzuki et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

HSV-TK, CS2-Venus

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.F102G.mRuby3

Plasmid

#112180

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

Tags

mRuby3

Expression

Mammalian

Mutation

F102G

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-ERas

Plasmid

#13355

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

ES cell expressed Ras (Eras Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
ss-cfSGFP2

Plasmid

#37535

Depositor

Ikuo Wada

Article

Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

Insert

ss-cfSGFP2 (cysteine-free SGFP2)

Use

Tags

signal sequence of α1-antitrypsin (M1-K34)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pF3BGX

Plasmid

#138387

Purpose

Empty RMCE vector (phiC31 attB/loxP)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

P5' FRT3 3xP3-Venus φC31-attB loxP FRT3 P3'

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX-mScarlet-I

Plasmid

#138391

Purpose

RMCE vector for generating C-terminal mScarlet-I fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

mScarlet-I

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Esg1

Plasmid

#13352

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

developmental pluripotency associated 5 (Dppa5a Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pF3BGX-T2A-GAL4

Plasmid

#138392

Purpose

RMCE vector for generating C-terminal T2A-GAL4 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Ecat1

Plasmid

#13351

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

ES cell associated transcript 1 (2410004A20Rik Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pT-35

Plasmid

#16092

Depositor

Yoshiko Kikuchi

Article

Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

Insert

SMT3 (SMT3 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pF3BGX-T2A-GAL80

Plasmid

#138396

Purpose

RMCE vector for generating C-terminal T2A-GAL80 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL80

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPyCAG-Myc-ERas-IP

Plasmid

#15690

Depositor

Shinya Yamanaka

Article

Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

Insert

ERas (Eras Mouse)

Use

Tags

Myc (6x)

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pMXs-EGFP-Rheb SSVM-IP

Plasmid

#13832

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb SSVM (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C181 changed to serine

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pCAG-ERas-IH

Plasmid

#15688

Depositor

Shinya Yamanaka

Article

Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

Insert

ERas (Eras Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 216C

Plasmid

#17523

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 1-215

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
iXFGFR1

Plasmid

#31257

Depositor

Masahiro Yamagishi

Article

Yamagishi et al Int J Dev Biol. 2010 . 54(1):93-104.

Insert

Xenopus FGFR1-ICD-Fv’Fv (fgfr1-a Frog)

Use

Tags

HA epitope tag and myristoylation signal

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N281

Plasmid

#17524

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 281 amino acids

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N391

Plasmid

#17525

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 391 amino acids

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pF3BGX-Venus

Plasmid

#138388

Purpose

RMCE vector for generating C-terminal Venus fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

Venus

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRIT Pax7 34C

Plasmid

#17522

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 1-33

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
p067 pCS mTRPS1 P4

Plasmid

#11039

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 P4 (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Truncation at aa1121 to mimic frameshift at codon…

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N33-170C

Plasmid

#17526

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 34-159 (paired domain)

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N215-273C

Plasmid

#17527

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 216-272 (homeodomain)

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pCCM937

Plasmid

#58982

Purpose

Expresses avr-15 sgRNA in C.elegans.

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

avr-15 sgRNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pCCM936

Plasmid

#58981

Purpose

Expresses avr-14 sgRNA in C.elegans.

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

avr-14 sgRNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pNHA8

Plasmid

#223650

Purpose

BccP protein purification

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

BccP

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

LacI

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pF3BGX-mTurquoise2

Plasmid

#138390

Purpose

RMCE vector for generating C-terminal mTurqouise2 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

mTurquoise2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
iXFGFR1k

Plasmid

#31259

Depositor

Masahiro Yamagishi

Article

Yamagishi et al Int J Dev Biol. 2010 . 54(1):93-104.

Insert

Xenopus FGFR1-ICD-FKBP (fgfr1-a Frog)

Use

Tags

HA epitope tag and myristoylation signal

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
iXFGFR1r

Plasmid

#31261

Depositor

Masahiro Yamagishi

Article

Yamagishi et al Int J Dev Biol. 2010 . 54(1):93-104.

Insert

Xenopus FGFR1-ICD-FRB (fgfr1-a Frog)

Use

Tags

HA epitope tag and myristoylation signal

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pMXs-Oct3/4

Plasmid

#13366

Purpose

Retroviral expression of mouse Oct3/4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.iGABASnFR

Plasmid

#112172

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Use

AAV

Tags

Expression

Mutation

Promoter

GFAP

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.hSynap.iGABASnFR

Plasmid

#112159

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has Service

AAV1

Insert

iGABASnFR

Use

AAV

Tags

Expression

Mutation

Promoter

hSynapsin

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.hSynap.iGABASnFR.F102G

Plasmid

#112160

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Tags

Expression

Mutation

F102G

Promoter

hSynapsin

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-mWatermelon

Plasmid

#190625

Purpose

Bacterial expression of mWatermelon GFP

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

mWatermelon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-puro GFP-b5 ER

Plasmid

#38274

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

Cytochrome b5 (Cyb5a Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Cyb5a Tranmembrane region from 95 to 134 amino ac…

Promoter

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only
pET41c-PhTET2

Plasmid

#182428

Purpose

Expresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coli

Depositor

Bruno Franzetti

Article

Gauto et al Nat Commun. 2022 Apr 8;13(1):1927. doi:

Insert

Tetrahedral aminopeptidase TET2 from P. hirokoshii

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-c-Myc

Plasmid

#13375

Purpose

Retroviral expression of mouse c-Myc

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (Myc Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pMH-EF1-GFP

Plasmid

#133018

Purpose

(Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.

Depositor

Michael Huen

Article

Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

Type

Empty backbone

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKI1.1R

Plasmid

#85808

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Expression

Plant

Mutation

Promoter

AtRPS5A

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKIR1.1

Plasmid

#85758

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG

Expression

Plant

Mutation

Promoter

AtRPS5A

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold

Plasmid

#186981

Purpose

mammalian expression of Cas7-11 crRNA guide

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

mature DR guide

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pF3BGX-T2A-GAL4-DBD

Plasmid

#138394

Purpose

RMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4-DBD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pF3BGX-7xGFP11

Plasmid

#138397

Purpose

RMCE vector for generating C-terminal fusion of seven tandem GFP11 tags (split GFP)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

7xGFP11

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-puro GFP-p62 D337, 338, 339A

Plasmid

#38280

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed D337A, D338A, D339A

Promoter

Available since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
pAAV.hSyn-FLEX.iGABASnFR

Plasmid

#112163

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has Service

AAV1

Insert

iGABASnFR

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Nano-lantern/pcDNA3

Plasmid

#51970

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-IP GFP-WIPI-1

Plasmid

#38272

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

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