We narrowed to 15,323 results for: pET
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Plasmid#127013PurposeBasic Part R- RBS; pET vector Ribosome Binding Site.DepositorInsertPart RBS-pET_R
UseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-petD 3'UTR
Plasmid#123571PurposeMoChlo kitDepositorInsertpetD 3'UTR
UseSynthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Wing
Plasmid#124552PurposeChimeric ETS domain: Wing of Ets-1 in PU.1 scaffoldDepositorTags6xHis tagExpressionBacterialMutationThe 5 residues making up the "wing" in …Available SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Loop
Plasmid#124551PurposeChimeric ETS domain: Loop of Ets-1 in PU.1 scaffoldDepositorTags6xHis tagExpressionBacterialMutationResidues 216 to 223 replaced with corresponding s…Available SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR E25R
Plasmid#119831PurposeProduction of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR E25R
TagsGSTExpressionBacterialMutationE25RPromoterT7Available SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-SOMA-T679A
Plasmid#118936PurposeUsed to express the T679A mutant form of the SOMA FRET sensor in E. coli.DepositorInsertSOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana with T679A Mutation
ExpressionBacterialAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes7/Imdes7
Plasmid#107115Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des7DepositorInsertcolEdes7/Imdes7
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes8/Imdes8
Plasmid#107116Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des8DepositorInsertcolEdes8/Imdes8
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes9/Imdes9
Plasmid#107117Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des9DepositorInsertcolEdes9/Imdes9
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes10/Imdes10
Plasmid#107118Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des10DepositorInsertcolEdes10/Imdes10
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes11/Imdes11
Plasmid#107119Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des11DepositorInsertcolEdes11/Imdes11
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes12/Imdes12
Plasmid#107120Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des12DepositorInsertcolEdes12/Imdes12
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes13/Imdes13
Plasmid#107121Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des13DepositorInsertcolEdes13/Imdes13
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes14/Imdes14
Plasmid#107122Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des14DepositorInsertcolEdes14/Imdes14
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes15/Imdes15
Plasmid#107123Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des15DepositorInsertcolEdes15/Imdes15
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes16/Imdes16
Plasmid#107124Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des16DepositorInsertcolEdes16/Imdes16
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes17/Imdes17
Plasmid#107125Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des17DepositorInsertcolEdes17/Imdes17
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes18/Imdes18
Plasmid#107126Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des18DepositorInsertcolEdes18/Imdes18
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes1/Imdes1
Plasmid#107108Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des1DepositorInsertcolEdes1/Imdes1
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes2/Imdes2
Plasmid#107109Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des2DepositorInsertcolEdes2/Imdes2
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes3/Imdes3
Plasmid#107110Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des3DepositorInsertcolEdes3/Imdes3
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes3.5/Imdes3.5
Plasmid#107111Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5DepositorInsertcolEdes3.5/Imdes3.5
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes4/Imdes4
Plasmid#107112Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des4DepositorInsertcolEdes4/Imdes4
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes5/Imdes5
Plasmid#107113Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des5DepositorInsertcolEdes5/Imdes5
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes6/Imdes6
Plasmid#107114Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des6DepositorInsertcolEdes6/Imdes6
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0387_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2
Plasmid#116999PurposeExpresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertRivularia sp. PCC 7116 RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0388_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2_RC
Plasmid#117000PurposeExpresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2 RC, compatible with SENECA acquisition readoutDepositorInsertRivularia sp. PCC 7116 RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0386_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1_RC
Plasmid#116998PurposeExpresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertRivularia sp. PCC 7116 RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0347_pET30-RT-CRISPR_Campylobacter_fetus_subsp._Fetus_ARRAY_1
Plasmid#116959PurposeExpresses CfRT-Cas1-Cas2 under pT7lac promoter, encodes CfCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertCampylobacter fetus subsp. Fetus RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0348_pET30-RT-CRISPR_Campylobacter_fetus_subsp._Fetus_ARRAY_1_RC
Plasmid#116960PurposeExpresses CfRT-Cas1-Cas2 under pT7lac promoter, encodes CfCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertCampylobacter fetus subsp. Fetus RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/68
Plasmid#113921PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68DepositorInsertMBP-NanoLucCP-65/68
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/66
Plasmid#113920PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66DepositorInsertMBP-NanoLucCP-65/66
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-LUCIDRS-MTX
Plasmid#113925PurposePlasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexateDepositorInsertLUCID_RS-MTX
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbI S178A
Plasmid#114159PurposeExpresses ClbI S178ADepositorInsertclbI
Tags6xHis and 6xHis-Thrombin siteExpressionBacterialMutationMutated S178 to AlaAvailable SinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-C-A2-PCP
Plasmid#114157PurposeExpresses ClbH-C-A2-PCPDepositorInsertclbH
Tags6xHis-thrombin-T7 tagExpressionBacterialMutationOnly contains the C-A2-PCP domains of ClbHAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
Tags6His-TEVExpressionBacterialMutationP46APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47A)
Plasmid#112722PurposeArg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R47A)
Tags6His-TEVExpressionBacterialMutationR47APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47K)
Plasmid#112723PurposeArg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.DepositorInsert6His-TEV-TBP6.7(R47K)
Tags6His-TEVExpressionBacterialMutationR47KPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48A)
Plasmid#112724PurposeGln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.DepositorInsert6His-TEV-TBP6.7(Q48A)
Tags6His-TEVExpressionBacterialMutationQ48APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
Tags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(T50A)
Plasmid#112727PurposeLav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.DepositorInsert6His-TEV-TBP6.7(T50A)
Tags6His-TEVExpressionBacterialMutationT50APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P51A)
Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
Tags6His-TEVExpressionBacterialMutationP51APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R52A)
Plasmid#112729Purpose52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.DepositorInsert6His-TEV-TBP6.7(R52A)
Tags6His-TEVExpressionBacterialMutationR52APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q54A)
Plasmid#112730PurposeAlthough not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.DepositorInsert6His-TEV-TBP6.7(Q54A)
Tags6His-TEVExpressionBacterialMutationQ54APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-CreM_E352A
Plasmid#112239PurposepET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM with E352A mutationDepositorInsertCreM_E352A
TagsMBPMutationGlutamate 352 (E352) mutated to an alanine.Available SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-C-SH2
Plasmid#111419Purposeexpress murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-C-SH2+IA
Plasmid#111418Purposeexpress murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
TagsHHHHHHENLYFQGExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2+IA
Plasmid#111273Purposeexpress murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
TagsHHHHHHENLYFQGExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RupA_T1-NHis
Plasmid#109259PurposeNRPS T domain with N-terminal 6His tagDepositorArticleInsertRupA_T1
TagsHis tagExpressionBacterialMutationcontains aa 917..1025 of WP_015523502.1PromoterT7Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only