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pAcGFP-Hyg-CTCF-Nterm-N1

Plasmid

#131800

Purpose

Expresses the [N-terminus of human CTCF (a.a. 2-265)]-GFP in mammalian cells

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

CTCF (CTCF Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 796-2181 (the N-terminus is l…

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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NC15 pGLUE CUL3

Plasmid

#36970

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

CUL3 (CUL3 Human)

Use

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2012

Availability

Academic Institutions and Nonprofits only
pLL5.0 mCherry-hCortKD-Flcortmut3YD

Plasmid

#187266

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

3YD (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD-Flcortmut3YF mCherry

Plasmid

#187265

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

3YF (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-SPAG5

Plasmid

#31217

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

SPAG5 (SPAG5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Contains a one nucleotide deletion at C-term that…

Promoter

Available since

Aug. 19, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

Plasmid

#70668

Purpose

Human mutant of E2F1 lacking amino acids 2 to 126

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 2 to 126 (E2F1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

lacks amino acids 2-126

Promoter

cmv

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-LC3-K51A

Plasmid

#155265

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

Lysine 51 to Alanine

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3-R70A

Plasmid

#155264

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

Arginine 70 to Alanine

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG

Plasmid

#74263

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

p.R404X truncation

Promoter

CMV

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only
pLL5.0 hCortKD-Flcortmut3YF EGFP

Plasmid

#187263

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

3YF (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_3

Plasmid

#103900

Purpose

Protein expression and purification of PTPN13 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MAGI1 (MAGI1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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MAGI1_PDZ_2

Plasmid

#103899

Purpose

Protein expression and purification of MAGI1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MAGI1 (MAGI1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-RanV19 E1RA2

Plasmid

#118317

Purpose

Expression of human Ran

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Inserts

RanV19 (RAN Human)
EPac1RA2 (RAPGEF3, RAPGEF4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

in-frame insertion of RanV19 into GFPEpac1RA2 and…

Promoter

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-RFP-YAP (isoform 5)

Plasmid

#235690

Purpose

Express RFP tagged YAP gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP32

Plasmid

#196770

Purpose

Contains RBOHF promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

RESPIRATORY BURST OXIDASE HOMOLOG F (RBOH F Mustard Weed)

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.EPC1.delta.EPcA

Plasmid

#184655

Purpose

Retroviral expression of mouse EPC1 delta EPcA

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc1 (Epc1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

epc1 delta EPcA

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag.Mm.Epc1-EPcA

Plasmid

#184657

Purpose

Retroviral expression of mouse Epc1 EPcA

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc1 (Epc1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Epc1-EPcA

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.EPC1.delta.EPcC

Plasmid

#184656

Purpose

Retroviral expression of mouse EPC1 delta EPcC

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc1 (Epc1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Epc1 delta EPCc.QT

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)

Plasmid

#175256

Purpose

SPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 Na

Plasmid

#124928

Purpose

For expression of a.a. 1-68 of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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