We narrowed to 3,314 results for: GCT

1. pCHW00016

   Plasmid

   #222447

   Purpose

   Level 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   p65AD-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458_CEBPB_1

   Plasmid

   #64036

   Purpose

   Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. GEARBOCS-SPARCL1 KO

   Plasmid

   #218182

   Purpose

   To KO Hevin in astrocytes

   Depositor

   Cagla Eroglu

   Article

   Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

   Insert

   sgRNA (Sparcl1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-MKL1/2 shRNA

   Plasmid

   #27161

   Purpose

   Lentiviral shRNA vector for knockdown of both mouse MKL1 and MKL2.

   Depositor

   Ron Prywes

   Article

   Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

   Insert

   shRNA MKL1+2
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. ACE2 g3

   Plasmid

   #153013

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

   Depositor

   Jason Sheltzer

   Insert

   ACE2 (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEPYC0CM0134

   Plasmid

   #162307

   Purpose

   Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   C-terminal tag, HiBiT-stop, frame1 (NNT-TCG)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

   Plasmid

   #118157

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-L3US2-RFP-HDAC3

   Plasmid

   #83966

   Purpose

   Lentiviral CRISPR HDAC3 dual gRNA targeting vector

   Depositor

   Eleanor Chen

   Insert

   HDAC3 dual gRNAs (HDAC3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MS2-pegRNA

   Plasmid

   #181811

   Purpose

   ms2 incorporated pegRNA-1.1

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

   Insert

   MS2-pegRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BCJJ180

    Plasmid

    #117516

    Purpose

    Cas9_4 Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_4:GCTT:BsaI
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICSL90016

    Plasmid

    #117514

    Purpose

    Cas9_2 Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_2:GCTT:BsaI
    

    Use

    CRISPR

    Tags

    NLS_SV40

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-Crispr-V2-HMID.v1

    Plasmid

    #103062

    Purpose

    LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

    Depositor

    Jay Shendure

    Article

    McKenna et al Science. 2016 May 26. pii: aaf7907.

    Insert

    Cas9 plus expressed GESTALT V1-V5 guide
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPYC0CM0257

    Plasmid

    #162308

    Purpose

    Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    C-terminal tag, HiBiT-stop, frame2 (TTC-G)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPQD0KN0285

    Plasmid

    #162290

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pColE1_sgRNA_pos5_AmpR

    Plasmid

    #119148

    Purpose

    Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 5 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPQD0CM0029

    Plasmid

    #162311

    Purpose

    Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    C-terminal tag, strep tag, stop codon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPQD0KN0284

    Plasmid

    #162289

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330.iGFP2

    Plasmid

    #66582

    Purpose

    sgRNA for iGFP

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    iGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pEPQD0CM0028

    Plasmid

    #162310

    Purpose

    Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    C-terminal tag, 9 aa linker, strep tag, stop codon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBSU6-shTOM40.2

    Plasmid

    #89882

    Purpose

    Expresses shRNA against TOM40

    Depositor

    Robert Friedlander

    Article

    Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

    Insert

    shRNA Tom40
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge