We narrowed to 1,758 results for: nif
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pPP394
Plasmid#158800PurposePlasmid containing Homo sapiens codon optimized CasΦ-1 and a SapI-GG stuffer spacer for editing in human cells.DepositorInsertCasPhi-1
ExpressionMammalianAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Csy4H29AS50C
Plasmid#45029DepositorInsertCsy4H29AS50C
TagsHis6-MBPExpressionBacterialMutationH29A mutation to abolish catalytic activity and S…PromoterT7Available SinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMJ824
Plasmid#39314DepositorInsertCas9
UseCRISPRTagsGST and His6ExpressionBacterialPromoterT7Available SinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPP076
Plasmid#158794PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-1
ExpressionBacterialAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-gRNA_BbsI_CAM
Plasmid#183222PurposeLbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertLbCas12a guide RNA containing BbsI sites in spacer sequence
UseCRISPRExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
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2xNLS-pMJ915v2
Plasmid#88916PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRExpressionBacterialAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a D832A
Plasmid#113517PurposeExpresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nucleaseDepositorInsertLbCas12a
TagsMBPExpressionBacterialMutationD832APromoterT7Available SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCJH027_SC101_pLAC_LbCas12a_Kan
Plasmid#183074PurposeWild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertLbCas12a
ExpressionBacterialMutationWild-typeAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
SiT-EGFP
Plasmid#166943Purposemammalian expression of N' Sialyl Transferase tagged with EGFPDepositorInsertSialyl Transferase N-terminus
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_083 Cas12i1
Plasmid#171670PurposeFor bacterial expression of Cas12i1 with an N-terminal His-MBP tagDepositorInsertCas12i1
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLBH546_Tet-Cas14b1Locus
Plasmid#112503PurposeExpresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14b1
UseCRISPRExpressionBacterialPromoterTetAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLBH547_Tet-Cas14b2Locus
Plasmid#112504PurposeExpresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14b2
UseCRISPRExpressionBacterialPromoterTetAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMJ915v2 + sfGFP
Plasmid#88918PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRExpressionBacterialAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_full-site
Plasmid#186277PurposeReporter plasmid for full-site selection assayDepositorInsertThiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
ExpressionBacterialAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a H759A
Plasmid#113518PurposeExpresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processingDepositorInsertLbCas12a
TagsMBPExpressionBacterialMutationH759AAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBA1032
Plasmid#189580PurposeT4soc deletion HR vector 250bp HR Arms (T4 phage editing)DepositorInsertT4soc Deletion Locus 250bp Homology Arms
UseUnspecifiedMutationEncodes for a full deletion of T4socAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Ppr_Cas13a_WT
Plasmid#91872PurposeBacterial expression for Cas13aDepositorInsertPprCas13a (PprC2c2) WT
TagsHis6-MBP-TEV siteExpressionBacterialAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
1xNLS-pMJ915v2
Plasmid#88915PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertCas9
UseCRISPRExpressionBacterialAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only
