We narrowed to 59,461 results for: %s

1. HBT-CPK32-Flag

   Plasmid

   #107432

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK32 (CPK32 Mustard Weed)
   

   Tags

   Flag

   Expression

   Bacterial

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKb-TnpB1

   Plasmid

   #215333

   Purpose

   ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

   Depositor

   Kutubuddin Molla

   Article

   Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

   Insert

   Rice codon optimized ISDra2 TnpB
   

   Tags

   Not applicable

   Expression

   Plant

   Promoter

   Rice ubiquitin promoter for TnpB and OsU3 promote…

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAnc80L65

   Plasmid

   #68837

   Purpose

   Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

   Depositor

   Luk Vandenberghe

   Article

   Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

   Insert

   Ancestral AAV Capsid Anc80L65
   

   Use

   AAV and Synthetic Biology

   Expression

   Mammalian

   Promoter

   Rep2

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HtrA2-FLAG

   Plasmid

   #15938

   Depositor

   L. Miguel Martins

   Article

   Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

   Insert

   HtrA2 (HTRA2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-GST-BcPC-PLC

   Plasmid

   #224255

   Purpose

   Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

   Depositor

   Chiaki Murakami

   Article

   Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

   Insert

   Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
   

   Tags

   GST and HRV-3C (LEVLFQGP) site

   Expression

   Bacterial

   Mutation

   The gene of Bacillus cereus ATCC 14579 was used f…

   Promoter

   lac promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. His6tev CBP HAT (1324-1700)

   Plasmid

   #99340

   Purpose

   bacterial expression of His tagged mouse CBP HAT domain (1324-1700)

   Depositor

   Peter Wright

   Article

   Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

   Insert

   mouse CBP HAT domain (1324-1700) (Crebbp Mouse)
   

   Tags

   His6tev

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Plasmid

   #100798

   Purpose

   helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G

   Depositor

   Ian Wickersham

   Article

   Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

   Has Service

   AAV1

   Insert

   TVA, EGFP, tet transactivator
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   synapsin

   Available Since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQCascade-array1

   Plasmid

   #140623

   Purpose

   Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Intersectin Long

   Plasmid

   #47395

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   intersectin long (ITSN1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti_CMV-Cas9-P2A-HygR

    Plasmid

    #164133

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQCascade-IS1

    Plasmid

    #140624

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-EglN2-pcDNA3

    Plasmid

    #18961

    Depositor

    William Kaelin

    Article

    Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

    Insert

    Egg laying Nine - 2 (EGLN2 Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pv6_CBX7_2xChromo

    Plasmid

    #179396

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_2xChromo
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    G113V in flexible linker

    Promoter

    CAGGS

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYDS2.0_AT118-H

    Plasmid

    #222846

    Purpose

    Yeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Expression

    Yeast

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

    Plasmid

    #198035

    Purpose

    Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    SINTBAD (TBKBP1 Human)
    

    Tags

    EGFP and GST-TEV

    Expression

    Insect

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CaMKII-EGFPKASH

    Plasmid

    #124882

    Purpose

    labelling of neuronal nuclear envelope with EGFP

    Depositor

    Vassilis Pachnis

    Article

    Obata et al Nature. 2020 Feb;578(7794):284-289. doi:

    Insert

    EGFP
    

    Use

    AAV

    Tags

    KASH

    Promoter

    CaMKII

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC001 - huLshC2C2-MBP for bacterial expression

    Plasmid

    #79150

    Purpose

    Expresses human codon-optimized LshC2c2 for purification in E. coli

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    C2c2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-myc-HLA-B7

    Plasmid

    #135509

    Purpose

    Mammalian expression of myc-tagged HLA-B*07:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*07:02 (HLA-B Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFLAG-Egfr(Velvet)

    Plasmid

    #18788

    Depositor

    Bruce Beutler

    Article

    Du et al Genetics. 2004 Jan . 166(1):331-40.

    Insert

    epidermal growth factor receptor (Egfr Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D833G; signal sequence removed (aa1-24)

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-Target-ACEmax

    Plasmid

    #139105

    Purpose

    Mammalian Target-ACEmax expression vector (pSI-625)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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