We narrowed to 58,610 results for: %s

1. pCEP4-myc-HLA-B15

   Plasmid

   #135513

   Purpose

   Mammalian expression of myc-tagged HLA-B*15:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-B*15:01 (HLA-B Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. ER-CanlonicSF

   Plasmid

   #178343

   Purpose

   To explore endoplasmic reticulum lactate dynamics

   Depositor

   Alejandro San Martín

   Article

   Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

   Insert

   ER-CanlonicSF
   

   Use

   Tags

   ER Retention Signal, ER Signal Exon 1, and ER Sig…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSW646 - pML1>>

   Plasmid

   #115995

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
   

   Use

   Tags

   HDEL and SP(ER)

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PLL5.0-Anillin ShRNA-Cherry-AnillinFL

   Plasmid

   #187271

   Purpose

   Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   U6, CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL5.0-Anillin shRNA

   Plasmid

   #187270

   Purpose

   Lentiviral expression of shRNA targeting Anillin

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   shRNA for Anillin (ANLN Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX_305-N-dTAG_OGT

   Plasmid

   #155196

   Purpose

   N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x HA and FKBP12(F36V)

   Expression

   Mammalian

   Mutation

   lacking N-terminal Met

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCX0393

   Plasmid

   #205984

   Purpose

   Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Inserts

   Ga0116245_10002768 candidate type VII proteins
   Cas14
   Cas5
   truncated CRISPR array
   

   Use

   Tags

   14xHis and TwinStrep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (proteins), pJ23119 (crRNA)

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cytosolic Fyn FRET Biosensor

   Plasmid

   #124654

   Purpose

   FRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

   Insert

   Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAFW-Ago2

   Plasmid

   #50554

   Purpose

   Expression of FLAG tagged Ago2 in S2 cell

   Depositor

   Yukihide Tomari

   Article

   Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

   Insert

   Argonaute2 (AGO2 Fly)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUltraI-Tet3.0[TAA]

    Plasmid

    #164580

    Purpose

    Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mb Tet3.0RS (R2-3)
    Mm tRNA[TAA]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L170G, N311G, C313A

    Promoter

    ProK and TacI

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHS1950

    Plasmid

    #205983

    Purpose

    Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Inserts

    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    CRISPR array
    

    Use

    Tags

    14xHis and TwinStrep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (proteins), pJ23119 (crRNA)

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ265E6

    Plasmid

    #213469

    Purpose

    Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

    Depositor

    Yiping Qi

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Insert

    TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
    

    Use

    CRISPR; Gateway compatible tada-cda-32aa linker-z…

    Tags

    No

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-ms-L-Myc-L351P

    Plasmid

    #50777

    Purpose

    retroviral expression of mouse L-Myc L351P mutant

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    L-Myc-L351P (Mycl Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L351P (does not bind to Max)

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR1A mKate2-hu_ncOGT WT

    Plasmid

    #154282

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    WT OGT

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-ASAP2s

    Plasmid

    #101276

    Purpose

    Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

    Depositor

    Francois St-Pierre

    Article

    Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

    Insert

    ASAP2s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn (human synapsin)

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI

    Plasmid

    #159756

    Purpose

    PiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backbone

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHelper-T7IS1

    Plasmid

    #140631

    Purpose

    Expresses ShCAST under the T7 promoter and an empty sgRNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. AsCas12a-2C-NLS in pCSDest

    Plasmid

    #126637

    Purpose

    Expresses AsCas12a-2C-NLS in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    AsCas12a-2C-NLS
    

    Use

    CRISPR

    Tags

    3xHuman influenza hemagglutinin (HA)-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94 promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO-puro-tetON-shGFP

    Plasmid

    #110939

    Purpose

    Tet-inducible shRNA targeting GFP

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shGFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-TRE-Gluc-RMA-IRES-EGFP

    Plasmid

    #189628

    Purpose

    Expresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and TRE

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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