We narrowed to 30,867 results for: PLE;

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pU6_(GLuc)_INT[3xPP7_SL]

Plasmid

#68424

Purpose

Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct bearing three PP7 Stem-loops

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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PERK 4: PERKKD-pGEX4T-1

Plasmid

#21817

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (537-1114) (Eif2ak3 Mouse)

Tags

GST and Myc (9E10)

Expression

Bacterial

Mutation

PERK Kinase Domain. Amino acids 537-1114.

Available Since

March 29, 2010

Availability

Academic Institutions and Nonprofits only

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P[acman]-Alpha2

Plasmid

#165859

Purpose

Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIH-PSM-Rb

Plasmid

#37106

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

Rb (RB1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

aa 379-928 only; large pocket of human Rb with aa…

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 GRK2 S670D

Plasmid

#45756

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 S670D (GRK2 Bovine)

Expression

Mammalian

Mutation

S670D

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
L2_UBE2:mT-N7-CsA

Plasmid

#136133

Purpose

Plasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:HygR p5-MpUBE2:mTurq-N7

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1-Nup35-mut

Plasmid

#87341

Purpose

To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP (F64L, S65T, V163A)-TRP1

Plasmid

#53184

Purpose

chromosomal tagging with stable GFP

Depositor

John Pringle

Article

Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP (F64L, S65T, V163A)

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-Centrin3

Plasmid

#136806

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin3

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIP Flag-hRpt6

Plasmid

#19793

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

hRpt6 (PSMC5 Human)

Use

Retroviral

Tags

Flag

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKII-VARNAM2

Plasmid

#195527

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CaMKII

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pBB75-ncRNA

Plasmid

#194087

Purpose

Expresses Ec86 ncRNA in Escherichia coli

Depositor

Tingting Zou

Article

Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

Insert

Ec86 ncRNA

Promoter

T7

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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PDLIM4_PDZ_1

Plasmid

#103941

Purpose

Protein expression and purification of RIL BGLII PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDLIM4 (PDLIM4 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG

Plasmid

#127300

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-STIL

Plasmid

#136844

Purpose

Mammalian expression of the centrosomal protein STIL N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-STIL (STIL Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHR TCF4

Plasmid

#16514

Depositor

Bert Vogelstein

Article

Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Insert

TCF4 (TCF7L2 Human)

Tags

Myc

Expression

Mammalian

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only
pISH-Gap43

Plasmid

#74336

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.

Depositor

Peter Mombaerts

Article

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

growth associated protein 43 (Gap43 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-Decoy

Plasmid

#80324

Purpose

Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

Decoy sgRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_hU6

Plasmid

#102843

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_hU6promoter

Use

As pcr template

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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MAC_N_CCDC93

Plasmid

#209687

Purpose

Expresses MAC-tagged CCDC93 in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

CCDC93 (CCDC93 Human)

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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