We narrowed to 59,496 results for: %s

1. TC1614

   Plasmid

   #164873

   Purpose

   CURE-X: C to U RNA Editing, with low off-target effects but also lower efficiency

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   hAPOBEC3A inserted into RfxCas13d (APOBEC3A )
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   human apobec Y132D

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1deltaART

   Plasmid

   #174804

   Purpose

   Bacterial expression of an inactive PARP1 mutant lacking the ART subdomain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1deltaART (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 785-1014

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Strep-His-Tev-hCGN

   Plasmid

   #236512

   Purpose

   For expression of Cingulin (human) in insect cells

   Depositor

   Sandra Citi

   Article

   Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

   Insert

   CGN (CGN Human)
   

   Tags

   2xStrep-10xHis-Tev

   Expression

   Insect

   Mutation

   WT

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ai34

   Plasmid

   #34881

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

   Insert

   CAG-Floxed Synaptophysin-tdTomato
   

   Use

   Mouse Targeting

   Tags

   tdTomato

   Promoter

   CAG

   Available Since

   March 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXS-hs-3xHA-LIN-41

   Plasmid

   #52717

   Purpose

   retroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Arrb1-RFP

   Plasmid

   #35401

   Depositor

   Robert Lefkowitz

   Article

   Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

   Insert

   beta-arrestin-1 (Arrb1 Rat)
   

   Tags

   RFP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-SYN-H-sDarken

   Plasmid

   #184797

   Purpose

   High affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   H-sDarken
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH-nCas9-PPE

   Plasmid

   #140447

   Purpose

   For plant prime editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   nCas9(H840A)-M-MLV
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   H840A for Cas9; D200N, T306K, W313F, T330P and L…

   Promoter

   maize Ubiquitin-1, OsU3

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Puro_siKO

   Plasmid

   #86696

   Purpose

   Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   H1 TO

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX317-VRK2

    Plasmid

    #199641

    Purpose

    Expresses VRK2

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK2 (VRK2 Human)
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFRT-DestFLAGHA_RBPMS

    Plasmid

    #65743

    Purpose

    constitutively expresses RBPMS in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCAGGS-RaichuEV-Rac1(Y40C)

    Plasmid

    #164903

    Purpose

    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    RaichuEV-Rac1 (RAC1 Human)
    

    Expression

    Mammalian

    Mutation

    Y40C mutation in the Rac1 domain whcih make the s…

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEL031

    Plasmid

    #137900

    Purpose

    STU2.0 SpyCas9 tRNA system for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Myc-HNF4A

    Plasmid

    #219396

    Purpose

    Express HNF4A in mammalian cells

    Depositor

    Yoichi Furukawa

    Article

    Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

    Insert

    HNF4A (HNF4A Human)
    

    Tags

    N terminal Myc tag

    Expression

    Mammalian

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB123 - pL0_rLUC-I (CDS1)

    Plasmid

    #123181

    Purpose

    Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
    

    Use

    Luciferase; Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites; codon-optimised for plants

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMZ7

    Plasmid

    #223180

    Purpose

    pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

    Depositor

    Paul Blainey

    Article

    Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

    Type

    Empty backbone

    Use

    Chromosomal integration

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. miniCGBE1-NG (pBM1182)

    Plasmid

    #140257

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_NG-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP

    Plasmid

    #232754

    Purpose

    Expresses EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    EGFP
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-hPhyB-mCherry-HRasCT

    Plasmid

    #100281

    Purpose

    Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (PHYB Mustard Weed)
    

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-908 aa)

    Promoter

    CAG promoter

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCl-HA-YAP

    Plasmid

    #27007

    Depositor

    Joan Massague

    Article

    Alarcon et al Cell. 2009 Nov 13. 139(4):757-69.

    Insert

    YAP (YAP1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only