We narrowed to 58,610 results for: %s

1. pInducer10-UBC9-sh4

   Plasmid

   #168988

   Purpose

   Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Ubiquitin Conjugating Enzyme 9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19.TRIM28.FKBP.knock-in.mCerulean

   Plasmid

   #185009

   Purpose

   For knocking in FKBP F36V P2A mCerulean

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   FKBP F36V P2A mCerulean
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-hs-3xHA-delta6xNHL-LIN-41

   Plasmid

   #52722

   Purpose

   retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   deleted amino acids 593–868

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-GFP-2AFP-KDEL

   Plasmid

   #154349

   Purpose

   Mammalian expression of 2AFP

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   2AFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-E6MBW6

   Plasmid

   #103102

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

   Plasmid

   #86611

   Purpose

   Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcFLAG-CSK-delta SH3 (73 to450 amino acids)

   Plasmid

   #74504

   Purpose

   Overexpression of CSK delta SH3 in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

   Insert

   CSK (CSK Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   del-SH3 (73 to450 amino acids)

   Promoter

   CMV

   Available since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pUDE710

   Plasmid

   #103020

   Purpose

   expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   crADE2-3.crHIS4-4.S (ADE2, HIS4 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. eGFP-KCC2

   Plasmid

   #104075

   Purpose

   expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

   Depositor

   Igor Medyna

   Article

   Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

   Insert

   KCC2 (b isoform) (Slc12a5 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EcoR1 site in rat KCC2 was removed by silent muta…

   Promoter

   CMV

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-M4-AR-S81D

    Plasmid

    #171242

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Asp (AR-S81D)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Ser81Asp mutant (AR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Ser81Asp (AR-S81D)

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1P DDX11_2

    Plasmid

    #160778

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1P DDX11_1

    Plasmid

    #160777

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSN25

    Plasmid

    #100110

    Purpose

    expresses a chimeric SOX2-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-SOX2 fused to full length HP1 alpha (SOX2, CBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSc-His6-Sem1Sc

    Plasmid

    #83235

    Purpose

    pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Sem 1 (SEM1 Budding Yeast)
    

    Use

    Tags

    His6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-mNRDc-E>A-V5

    Plasmid

    #51298

    Purpose

    expression of the inactive mutant of mouse NRDc in mammalian cells

    Depositor

    Eiichiro Nishi

    Article

    Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

    Insert

    NRDc E247A (Nrd1 Mouse)
    

    Use

    Tags

    HIS and V5

    Expression

    Mammalian

    Mutation

    E247A, enzymatic inactive mutant

    Promoter

    CMV

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pKI-cjFOXP2-hum

    Plasmid

    #129757

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    FOXP2 (LOC100411307 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    T301N/N323S

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEos3-dnRac1

    Plasmid

    #92040

    Purpose

    Expresses mEos3 fusion with dnRac1 (T17N).

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T17N (dominant negative)

    Promoter

    CMV

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-tetpA-hNEUROG2-iRPT

    Plasmid

    #140764

    Purpose

    Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

    Inserts

    iRFP713
    PuroR
    rtTAM2
    NEUROG2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGLS3-5xHRE-PHLDA3(wt)

    Plasmid

    #72557

    Purpose

    Hypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    PHLDA3 (PHLDA3 Human)
    

    Use

    Tags

    5XHRE

    Expression

    Mammalian

    Mutation

    Promoter

    E1b minimal

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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