We narrowed to 2,386 results for: PRK

1. PKN1 gRNA (BRDN0001149250)

   Plasmid

   #76275

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PKN1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PKN1 (PKN1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PKN1 gRNA (BRDN0001148556)

   Plasmid

   #76277

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PKN1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PKN1 (PKN1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLentiPGK Puro DEST p38KTRClover

   Plasmid

   #59152

   Purpose

   Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR SFFVp BFP-Erk D319N

   Plasmid

   #102350

   Purpose

   Lentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutation

   Depositor

   Jared Toettcher

   Article

   Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

   Insert

   Erk2 (Mapk1 Mouse)
   

   Use

   Lentiviral

   Tags

   BFP

   Expression

   Mutation

   Aspartic acid 319 was mutated to asparagine

   Promoter

   SFFV

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

   Plasmid

   #220493

   Purpose

   To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-RIIα-μDpTT

   Plasmid

   #182327

   Purpose

   PKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   RIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)
   

   Use

   Tags

   Full-length human PKA type 2alpha regulatory subu…

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWZLblasti-HA-ERK2 GOF

   Plasmid

   #53174

   Purpose

   Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Mustard Weed, Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R67S/D321N

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pGEX4T3-HA-ERK2 K54R

   Plasmid

   #53170

   Purpose

   Expresses GST-HA tagged human ERK2 K54R from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Mustard Weed, Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   K54R

   Promoter

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pWZLblasti-HA-ERK2 R67S

   Plasmid

   #53172

   Purpose

   Expresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Mustard Weed, Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R67S

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pWZLblasti-HA-ERK2 D321N

    Plasmid

    #53173

    Purpose

    Expresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Mustard Weed, Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D321N

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCMV5 Flag p38 alpha(agf)

    Plasmid

    #20787

    Depositor

    Roger Davis

    Article

    Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

    Insert

    pCMV5 Flag p38 alpha (agf) (Mapk14 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced Thr180 and Tyr182 with Ala and Phe, resp…

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pFUGW-shRIIα-RIIαWT-IRES-EGFP

    Plasmid

    #183456

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-sgAMPKa1-Cas9-GFP

    Plasmid

    #208049

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa1

    Depositor

    Naama Kanarek

    Article

    Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

    Insert

    sgPRKAA1 (PRKAA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

    Plasmid

    #183457

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

    Plasmid

    #183458

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCMV Puro DEST p38KTRmCerulean3

    Plasmid

    #59155

    Purpose

    Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-HA_N-mouse Bcl6

    Plasmid

    #138410

    Purpose

    Express mouse Bcl6 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

    Insert

    B cell lymphoma 6 (Bcl6 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-Puro-sgAMPKa2

    Plasmid

    #138685

    Purpose

    Expresses a human AMPKa2-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgAMPKa2 human (PRKAA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LMP AMPK alpha1 hp1

    Plasmid

    #89489

    Purpose

    shRNA against AMPK alpha1 in mouse/human to knock down protein expression

    Depositor

    Russell Jones

    Article

    Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

    Insert

    hairpin against mouse/human AMPKalpha1 (PRKAA1, Prkaa1 Mouse, Human)
    

    Use

    Mouse Targeting and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (The GFP-Puro is from a PGK promoter; the shRN…

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

20. MAPK14 gRNA (BRDN0001145809)

    Plasmid

    #77923

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK14

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK14 (MAPK14 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge