We narrowed to 15,270 results for: grn

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ULK1 gRNA (BRDN0001145044)

Plasmid

#75736

Purpose

3rd generation lentiviral gRNA plasmid targeting human ULK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ULK1 (ULK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ULK1 gRNA (BRDN0001145850)

Plasmid

#75737

Purpose

3rd generation lentiviral gRNA plasmid targeting human ULK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ULK1 (ULK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EGFR gRNA (BRDN0001146570)

Plasmid

#75688

Purpose

3rd generation lentiviral gRNA plasmid targeting human EGFR

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFR (EGFR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRPR1(1xTetO)_gRNA_handle_RPR1t

Plasmid

#62966

Purpose

aTc inducible gRNA expression vector

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Expression

Yeast

Promoter

RPR1

Available since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSTBlue-U6-sgRNA(tdTomato)

Plasmid

#47871

Purpose

This plasmid contains expression cassette for sgRNA targeting tdTomato.

Depositor

Erik Sontheimer, James Thomson

Publication

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

sgRNA-tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FUGW-H1-Scrambled control shRNA

Plasmid

#40625

Depositor

Sally Temple

Publication

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Scrambled control shRNA

Use

Lentiviral and RNAi

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSIRIP-puro shRNA p19-2

Plasmid

#14090

Depositor

Tyler Jacks

Publication

Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

Insert

p19 ARF shRNA -2 (Cdkn2a Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSuperRetropuro-IL-6 shRNA 1

Plasmid

#19959

Depositor

Christopher Counter

Publication

Ancrile et al Genes Dev. 2007 Jul 15. 21(14):1714-9.

Insert

pSuperRetropuro-IL-6 shRNA 1 (IL6 Human)

Use

RNAi and Retroviral

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFREE_Zeo

Plasmid

#92053

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Publication

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSP618

Plasmid

#169235

Purpose

Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

Depositor

William Greenleaf

Publication

Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001487130)

Plasmid

#80262

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJA42

Plasmid

#59930

Purpose

gRNA for cleavage at rol-6(su1006) locus in C elegans

Depositor

Andrew Fire

Publication

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rol-6(su1006) gRNA

Use

CRISPR

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PEmax-mRNA

Plasmid

#204472

Purpose

plasmid for PEmax mRNA IVT

Depositor

Scot Wolfe

Publication

Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Prime editor

Use

CRISPR

Tags

no

Expression

Mammalian

Promoter

T7

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHLS-EF1a-FRB-SpCas9-A

Plasmid

#138477

Purpose

Expresses FRB fused SpCas9 for NanoMEDIC packaging.

Depositor

Akitsu Hotta

Publication

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9, human codon optimized

Use

CRISPR

Tags

3x HA tag and FRB

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#162334

Purpose

Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

Depositor

Charles Gersbach

Publication

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSaCas9-KRAB

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hUbC

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-sgp53/Cre

Plasmid

#89646

Purpose

Expresses a p53-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Publication

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting p53 (Trp53 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO_FOXA1_#2

Plasmid

#70096

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Publication

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

u6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PL-5LTR-RGR(DMD#1)-AmCyan-A

Plasmid

#138482

Purpose

Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

Depositor

Akitsu Hotta

Publication

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ribozyme-flanked gRNA and AmCyan

Use

CRISPR

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g3

Plasmid

#80786

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

Depositor

Yonglun Luo

Publication

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin2a KI

Plasmid

#131486

Purpose

Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor (Grin2a Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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