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pME-H2B-V5-n2StayGoldC4 (JDW 1354)

Plasmid

#224486

Purpose

Gateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

H2B-V5-n2StayGoldC4

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}

Plasmid

#201120

Purpose

RFP expression in cells under EF-1α promoter; Histone-bound, nuclear

Depositor

Ori Bar-Nur

Article

Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

Insert

TurboRFP; H2B-RFP

Use

Lentiviral

Tags

Expression

Mutation

No

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.dEZH2

Plasmid

#220241

Purpose

Expression of an EZH2 catalytic site mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

dEZH2 (EZH2 Human)

Use

Tags

6x His, MBP

Expression

Insect

Mutation

Y731F

Promoter

Polyhedrin

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-p300 (D1399Y)

Plasmid

#191763

Purpose

Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

FRB-EGFP-p300 core (D1399Y) (EP300 Human)

Use

Tags

EGFP (N-terminal of p300 core (D1399Y)) and FRB (…

Expression

Bacterial and Mammalian

Mutation

catalytic D1399Y mutation in p300

Promoter

EF1alpha

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2-mt2

Plasmid

#220245

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

EF-1α

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET22-His-TEV-DOT1L

Plasmid

#89610

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

Depositor

Leemor Joshua-Tor

Article

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L (DOT1L Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2-mt2*

Plasmid

#220246

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2* (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

PRKKKR494-499NAAIRS

Promoter

EF-1α

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAG109 H2B-FAST

Plasmid

#130722

Purpose

Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

H2B-FAST

Use

Tags

myc-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET22-His-TEV-DOT1L-MM

Plasmid

#89611

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

Depositor

Leemor Joshua-Tor

Article

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L VVEL293MM Mutant (DOT1L Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-CHD5-Y619E-FRAG

Plasmid

#68871

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa, Y619E

Promoter

CMV

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-CHD5-WT-331-672aa

Plasmid

#68870

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa)

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa

Promoter

CMV

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
pHIV-EGFP.Flag-EZH2-mt1

Plasmid

#220244

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 1 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R27A, R31A, F165A

Promoter

EF-1α

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EZH2-mt2

Plasmid

#220240

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Use

Tags

6x His, MBP

Expression

Insect

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

Polyhedrin

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EZH2-mt1

Plasmid

#220239

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 1 (EZH2 Human)

Use

Tags

6x His, MBP

Expression

Insect

Mutation

R27A, R31A, F165A

Promoter

Polyhedrin

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LANS1-Set2

Plasmid

#122002

Purpose

FLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

Depositor

Brian Kuhlman

Article

Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

Insert

histone methyltransferase SET2 (SET2 Budding Yeast)

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Set2 point mutations K538G, K539S, R549G, K550S, …

Promoter

ADH1

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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DOT1L-CC3

Plasmid

#111754

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Use

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #3 (residues 613-661)

Promoter

T7

Available since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC1

Plasmid

#111756

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Use

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

contains coiled coil #1 (residues 506-555)

Promoter

T7

Available since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC2

Plasmid

#111755

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Use

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #2 (residues 560-608)

Promoter

T7

Available since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-PIG-N3FLAG-TAF12(50-130)

Plasmid

#105595

Purpose

retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

truncation fragments containing aa (50-130), and …

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-K36M TAIL[1-44]-3AID-HA-2A-mCherryBSD

Plasmid

#225872

Purpose

Lentiviral expression of AID degron tagged H3.3(K36M) histone tail corresponding to amino acids 1-44 and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3(K36M) amino acids 1-44 (H3-3A, H3-3B Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Mutation

amino acids 1-44 from H3.3 K36M

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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