162,309 results

1. EFNB1-bio-His

   Plasmid

   #51749

   Purpose

   Expresses full-length Ephrin-B1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   EFNB1 (EFNB1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-PhoCl1-6His

   Plasmid

   #164033

   Purpose

   Expressed PhoCl1 in a pET expression vector

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   PhoCl1
   

   Use

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHD-DsRed

   Plasmid

   #51434

   Purpose

   Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

   Depositor

   Kate O'Connor-Giles

   Article

   Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

   Insert

   LoxP-3xP3-DsRed-LoxP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   3xP3

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-TetOne-Puro-p21

   Plasmid

   #171122

   Purpose

   Doxycycline inducible expression of p21

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   p21 (CDKN1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-puro U6 sgRNA BfuAI stuffer

   Plasmid

   #50920

   Purpose

   U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-ALK5 wt

   Plasmid

   #80876

   Purpose

   mammalian expression of ALK5 WT

   Depositor

   Aristidis Moustakas

   Article

   Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

   Insert

   ALK5 (TGFBR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBJ1

   Plasmid

   #167130

   Purpose

   A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Frr promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRE reporter backbone

   Plasmid

   #158678

   Purpose

   This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG:H2B-EGFP

   Plasmid

   #32599

   Depositor

   Virginia Papaioannou, Anna-Katerina Hadjantonakis

   Article

   Hadjantonakis et al BMC Biotechnol. 2004 Dec 24;4:33.

   Insert

   H2B-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

10. EB3N-VVDfast-Halo-iLID

    Plasmid

    #190166

    Purpose

    Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    EB3N-VVDfast-Halo-iLID
    

    Use

    Tags

    -VVDfast-mClover3-iLID

    Expression

    Mammalian

    Mutation

    1-189 aa from EB3

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEP4 E02S EN2K

    Plasmid

    #20925

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Nanog and Klf4 (SOX2, POU5F1, NANOG, KLF4 Human)
    

    Use

    Episomal/ebna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXD054 KIKO CD19 CAR AAV vector

    Plasmid

    #192157

    Purpose

    CD19 CAR AAV vector

    Depositor

    Sidi Chen

    Article

    Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

    Insert

    anti-CD19 CAR (CD19 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-hTipin (pc4732)

    Plasmid

    #216862

    Purpose

    Mammalian expression vector encoding GFP tagged human Tipin

    Depositor

    Cristina Cardoso

    Article

    Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

    Insert

    Tipin (TIPIN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 IGF1R-BirA(R118G)-HA

    Plasmid

    #109232

    Purpose

    This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

    Depositor

    Victor Dzau

    Article

    Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

    Insert

    IGF1R (IGF1R Human)
    

    Use

    Tags

    BirA(R118G) (highly promiscuous form) and HA

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-EWSR1 Stop

    Plasmid

    #84887

    Purpose

    DONOR vector for Gateway cloning of EWSR1

    Depositor

    Aaron Gitler

    Article

    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Gateway donor

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. hSyn-somBiPOLES-mCerulean

    Plasmid

    #154945

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. 6xHis-TagRFP-T_A1aY1

    Plasmid

    #158754

    Purpose

    Expression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    6xHistidine_TagRFP-T_A1aY1
    

    Use

    Tags

    6x-Histidine tag and TagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA-3xHA-Reptin

    Plasmid

    #51636

    Purpose

    Mamamlian expression of 3xHA tagged reptin, N-terminal.

    Depositor

    Steven Artandi

    Article

    Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

    Insert

    reptin (RUVBL2 Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRetroX-Tight_A-CREB_PGK-tdTomato

    Plasmid

    #154264

    Purpose

    Dox/Tet-inducible expression of dominant negative CREB (A-CREB) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    dominant negative CREB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight/tetO

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CL20_mEGFP_ETV6_NTRK3

    Plasmid

    #205817

    Purpose

    Express mEGFP-tagged fusion protein, ETV6_NTRK3 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    ETV6_NTRK3 (ETV6, NTRK3 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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