We narrowed to 22,193 results for: TES

1. pDIV537

   Plasmid

   #177704

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Promoter

   RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S

   Plasmid

   #62460

   Purpose

   expresses C151S and C288S double mutant human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Tags

   His-Halo-Tev

   Expression

   Mammalian

   Mutation

   Changed Cysteines 151 and 288 of Keap1 to respect…

   Promoter

   CMV

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-shSNAI1

   Plasmid

   #115467

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   shSNAI1 (SNAI1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(TF)

   Plasmid

   #230001

   Purpose

   Expresses anti-TNFalpha PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   IL2SP-Aro6-Ozoralizumab-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   V360A/R361A on KORD

   Promoter

   CMV

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-anti-CCL2(8E10) PAGER(TF)

   Plasmid

   #230002

   Purpose

   Expresses anti-CCL2 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   IL2SP-Aro6-8E10-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   V360A/R361A on KORD

   Promoter

   CMV

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-anti-mCherry(LaM6) PAGER(TF)

   Plasmid

   #229998

   Purpose

   Expresses anti-mCherry PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   IL2SP-Aro6-LaM6-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   V360A/R361A on KORD

   Promoter

   CMV

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-eGFP_mito

   Plasmid

   #221414

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   eGFP_mito
   

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P-1-SF2

   Plasmid

   #99020

   Purpose

   Bacterial expression of SF2

   Depositor

   Honglin Chen

   Article

   Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

   Insert

   SF2 (SRSF1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-IRES-miR34a-P2

   Plasmid

   #64786

   Purpose

   Luciferase expression driven by miR-34a promoter P2

   Depositor

   Joshua Mendell

   Article

   Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

   Insert

   miR-34a promoter P2 (MIR34A Human)
   

   Use

   Luciferase

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-SP140

    Plasmid

    #65394

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SP140 (SP140 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pEF1a_FL MUT TET1

    Plasmid

    #124081

    Purpose

    Expression of catalytic inactive form of full-length TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - full length, mutated (TET1 Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1672Y, D1674A

    Promoter

    EF1 alpha

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Dnase1L3 R206C pIRES-DsRed

    Plasmid

    #98847

    Purpose

    Expresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRed

    Depositor

    Peter Keyel

    Article

    Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

    Insert

    Dnase1L3 (DNASE1L3 Human)
    

    Tags

    IRES DsRed

    Expression

    Mammalian

    Mutation

    R206C which greatly reduces nuclease activity

    Promoter

    CMV

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF709-pET28a-Hs-FL-ETFbeta-NHis wt

    Plasmid

    #85110

    Purpose

    expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length ETFbeta (ETFB Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-Mfn1-E209A

    Plasmid

    #141155

    Purpose

    Mammalian expression and labeling of mutant mitofusin 1 (E209A) deficient in GTP hydrolysis

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    MFN 1 (MFN1 Human)
    

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    changed Glutamic acid at position 209 to Alanine …

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. B*40:01:01

    Plasmid

    #203257

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*40:01:01 (HLA-B Human)
    

    Expression

    Mammalian

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTSlb-N748D

    Plasmid

    #60732

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR8/GW_TOPO_Cdk12_Untagged

    Plasmid

    #127176

    Purpose

    Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    None

    Promoter

    None

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. XIAP (pBS0852)

    Plasmid

    #185282

    Purpose

    For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    XIAP (XIAP Human)
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-delta133HtrA-EGFP

    Plasmid

    #14124

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the first 133 amino-acids

    Available Since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pCS2-mkgDUX4

    Plasmid

    #21156

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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