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We narrowed to 19,359 results for: MUT

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Showing: 11401 - 11420 of 19359 results

  2. pKM197

    Plasmid
    #132665
    Purpose
    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
    Depositor
    Joseph Sorg
    Article
    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:
    Insert
    Upstream & Downstream pyrE deletion region
    Use
    E. coli - c. difficile shuttle vector
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132665-plasmid-map-sequence-id-259679

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  5. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  6. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid
    #20297
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available Since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    20297-plasmid-map-sequence-id-354478

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  16. pAAV-CaMKIIa-hM4D(Gi)-mCherry

    Plasmid
    #50477
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry (CHRM4 Human)
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50477-plasmid-map-sequence-id-370683

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  19. pGPΩTp

    Plasmid
    #130660
    Purpose
    Insertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.
    Depositor
    Miguel Valvano
    Article
    Flannagan et al Infect Immun. 2007 Apr;75(4):1679-89. Ep
    Insert
    Ω trimethoprim cassette
    Expression
    Bacterial
    Mutation
    Addition of Ω sites next to trimethoprim cassette…
    Promoter
    dhfr promoter
    Available Since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    130660-plasmid-map-sequence-id-258172

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  20. pALS3-Ma-sfGFP-TAG150

    Plasmid
    #212121
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212121-plasmid-map-sequence-id-444186

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Showing: 11401 - 11420 of 19359 results