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pRK5 CASTOR1-Flag Cterm

Plasmid

#84488

Purpose

overexpression of CASTOR1 with Flag tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 (GATSL3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pALPS rtTA3_V14

Plasmid

#101350

Purpose

Codon optimized rtTA3 used in trans with Tet inducible HIV-1

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

rtTA3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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dCas12Max-ABE

Plasmid

#195335

Purpose

vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

human codon-optimized dCas12Max-ABE

Expression

Mammalian

Mutation

N243R, E336R, D1049A

Promoter

CBh, CMV, hU6

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet-1∆HisTag_hNMT_Nef

Plasmid

#66078

Purpose

Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

Depositor

Dieter Willbold

Article

Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

Inserts

NMT (NMT1 Human)
Nef

Expression

Bacterial

Mutation

Deletion of first 80 amino acids

Promoter

T7-lacO

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsXRN1

Plasmid

#66596

Purpose

expresses lambdaN-HA-tagged XRN1 in mammalian cells

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

XRN1 (XRN1 Human)

Tags

lambdaN-HA

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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H517 SONIC HRASV12 donor

Plasmid

#138177

Purpose

CRISPR SONIC: HRAS G12V donor plasmid

Depositor

Wen Xue

Article

Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

Insert

HRASV12 (HRAS Human)

Use

Nhej donor

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pZE27GFP

Plasmid

#75452

Purpose

Plasmid for constitutive expression of gfp

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp

Expression

Bacterial

Promoter

placIq promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC4A2_STOP

Plasmid

#161442

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A2 (SLC4A2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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modRNAc0-ABE8e-P2A-GFP

Plasmid

#178177

Purpose

IVT template plasmid for making ABE8e-P2A-GFP modRNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

ABE8e

Use

Ivt template plasmid

Tags

P2A-GFP

Promoter

T7 promoter

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP1339

Plasmid

#119271

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLEGFP-WT-STAT3

Plasmid

#71450

Purpose

Retroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV-

Plasmid

#101337

Purpose

Repaired U3 allows LTR-based transcription by the provirus with GFP as a marker for expression. WPRE was deleted.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Td-CGBE

Plasmid

#196599

Purpose

Mammalian expression of Td-CGBE

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS

Tags

6*His

Expression

Mammalian

Mutation

TadA-8e (N46L); S. pyogenes Cas9 (D10A)

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP CMV VHHG4

Plasmid

#182236

Purpose

Expresses GFP nanobody for stable expression in mammalian cells

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

anti-GFP nanobody

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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plRL2

Plasmid

#163631

Purpose

Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-AMIGO2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156803

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

AMIGO2 (AMIGO2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA Ef1alpha Puro-T2A-GFP

Plasmid

#111596

Purpose

sgRNA Ef1alpha Puro-T2A-GFP

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17

Plasmid

#210020

Purpose

tetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Klf4-IRES-Sox2-17 (Klf4, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO-mini-CMV

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-MAP

Plasmid

#66999

Purpose

Expresses malE-MAP in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-MAP

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV4-ApoE2

Plasmid

#87085

Purpose

Expresses ApoE2 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

Apolipoprotein E2 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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