165,775 results

1. pDEST17-UbE2R2

   Plasmid

   #15806

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. B-gTEMP/pcDNA3

   Plasmid

   #188447

   Purpose

   B-gTEMP for mammalian expression

   Depositor

   Takeharu Nagai

   Article

   Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

   Insert

   B-gTEMP
   

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIntegrase_8

   Plasmid

   #60579

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_8
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-SisTnpB1–ωRNA

   Plasmid

   #228365

   Purpose

   Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

   Depositor

   Tao Liu

   Article

   Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

   Insert

   SisTnpB1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMIR-FLAG-MLL-AF9

   Plasmid

   #71444

   Purpose

   Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

   Depositor

   Daisuke Nakada

   Article

   Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

   Insert

   MLL-AF9 (KMT2A Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR1A no ccDB (w48-1)

   Plasmid

   #17398

   Purpose

   Gateway entry 1A vector, no ccDB gene

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Entry vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFPC1-hVAP-A

   Plasmid

   #104447

   Purpose

   Expression of human VAP-A fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein A (VAPA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. ins:CFP-NTR

   Plasmid

   #66593

   Purpose

   Specific and conditional cell ablation of beta cells in the zebrafish pancreas

   Depositor

   Didier Stainier

   Article

   Curado et al Dev Dyn. 2007 Apr . 236(4):1025-35.

   Insert

   Insulin promotor driving CFP-nitroreductase
   

   Use

   Zebrafish expression

   Tags

   CFP fused with N-terminus of nitroreductase

   Promoter

   Zebrafish Insulin promoter (approximately 1.2kb)

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pBBR15.2-2H12.D11opt

   Plasmid

   #221185

   Purpose

   cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen2.1
   

   Expression

   Bacterial

   Promoter

   Constitutive

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe_puro_DEST_Flag_SKIL

    Plasmid

    #45265

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SNON (SKIL Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pAG Lenti CMV Empty Puro

    Plasmid

    #236083

    Purpose

    Empty lentiviral backbone

    Depositor

    Melina Fan

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    April 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pRCC-K

    Plasmid

    #81191

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-HA-Flag-natT1R2

    Plasmid

    #113944

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-N-Solo-mStayGold-TUBA1B

    Plasmid

    #227327

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid

    #104567

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    

    Use

    CRISPR

    Tags

    NLS-3xHA-VPR

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. epB_UbC_CymRV5-nls-GFP_DEx2

    Plasmid

    #119906

    Purpose

    ePiggyBac mammalian expression plasmid for CymR-GFP fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    CymRV5-nls-GFP
    

    Expression

    Mammalian

    Available Since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6a:sgRNA#2

    Plasmid

    #64246

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. mTagBFP2-Lysosomes-20

    Plasmid

    #55308

    Purpose

    Localization: Lysosome Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    LAMP1
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pISH-Msx1

    Plasmid

    #84313

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Msx1. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    Msx1 (Msx1 Mouse)
    

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-ERK2

    Plasmid

    #118296

    Purpose

    Expression of Rat ERK2

    Depositor

    Philip Stork

    Article

    Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

    Insert

    ERK2 (Mapk1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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