We narrowed to 66,633 results for: cell;

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CD8-mCherry-FRB

Plasmid

#100738

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD8A (CD8A Human)

Tags

FRB and mCherry

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-K298M

Plasmid

#140315

Purpose

Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

K298M

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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EK0313 SFFV-mCherry-SG(s70587)SG-Cre (FLP-IN)

Plasmid

#191149

Purpose

Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, Cre output derived from a gift from prof. Liqun Luo

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:Cre

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMMLV-mRFP1-P2A-Puro

Plasmid

#160228

Purpose

Expresses mRFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

mRFP1-P2A-Puro

Use

Retroviral

Expression

Bacterial and Mammalian

Promoter

LTR

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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betaTrCP mVenus reporter

Plasmid

#192458

Purpose

Fluorescent reporter for beta-TrCP activity in human cells

Depositor

Steven Cappell

Article

Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

Insert

CDC25B (CDC25B Human)

Use

Lentiviral

Tags

SV40 NLS and mVenus

Expression

Mammalian

Mutation

aa198-338

Promoter

EF1alpha

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-FoxG1

Plasmid

#35547

Depositor

Marius Wernig

Article

Lujan et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

FoxG1 (Foxg1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

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pcFLAG-CSK-WT

Plasmid

#74502

Purpose

Overexpression of CSK in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Tags

FLAG

Expression

Mammalian

Mutation

K222R

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
5'-BirA-FLAG GFP minimal CMV pCDNA5

Plasmid

#212087

Purpose

LR vector for integration of GFP into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

GFP

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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CLC-EGFP

Plasmid

#193566

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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RSV-Flag-Brd3

Plasmid

#86615

Purpose

Flag tagged mouse Brd3 expression construct for cell transfection

Depositor

Mario García-Domínguez

Article

Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

Insert

Brd3 (Brd3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

RSV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MTDH-His-bio

Plasmid

#52331

Purpose

Expresses full-length Metastasis adhesion protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

MTDH (MTDH Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-BAF_L58R

Plasmid

#101776

Purpose

Expresses EGFP tagged mutant BAF (L58R) in human cells

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

L58R mutation

Promoter

EF1a

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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ECibN

Plasmid

#209670

Purpose

Expresses E-cadherin fused with eGFP, CIB1, and Split-TurboID-N in mammalian cells

Depositor

Sanjeevi Sivasankar

Article

Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

Insert

E-Cadherin (CDH1 Canis lupus familiaris)

Tags

CIB1, Split-TurboID-N, and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV-PGKpuro2ABFP

Plasmid

#122372

Purpose

Expresses puro-2A-BFP in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

Puro-2A-BFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-Y530F

Plasmid

#140319

Purpose

Expresses Flag-tagged human Src-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

Y530F

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144[3072 nt]~451-CMV2-mir1

Plasmid

#164682

Purpose

Express miR-144[3072 nt]~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144[3072 nt]~451 (MIR144 Human)
mir-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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HA-UVRAG

Plasmid

#86738

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Tags

JA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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NLS-AgDD

Plasmid

#80625

Purpose

create nuclear protein aggregates in mammalian cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NLS-NES-L106P-sfGFP (FKBP1A Human)

Tags

MPPKKKRKVLALKLAGLDI

Expression

Mammalian

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Grb10-WT

Plasmid

#74501

Purpose

Overexpression of Grb10 in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Mol Oncol. 2013 Jun;7(3):402-18. doi: 10

Insert

Grb10 (GRB10 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L358S, V522A, and V524L

Promoter

CMV

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP

Plasmid

#121448

Purpose

To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations

Depositor

Brian Chen

Article

Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

Insert

RFPnols-PQR-ZeocinR (ACTB Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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