We narrowed to 24,993 results for: Nes

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pET28a-ybbR-HIS-sfGFP-DocI

Plasmid

#58708

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

sfGFP-DocI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE3 CTF-FLAG

Plasmid

#217691

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE3 (ADGRE3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-338

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE2 CTF-FLAG

Plasmid

#217690

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE2 (ADGRE2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-517

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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Twist-siRNA5

Plasmid

#8456

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siTwist (Twist1 Mouse)

Expression

Mammalian

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
attB53-Pac-attB53

Plasmid

#183610

Purpose

RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

Pac

Use

Rmce

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pR6Kg-Omega1

Plasmid

#165864

Purpose

Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP Δnef/U3

Plasmid

#101346

Purpose

Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta nef/LTR

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pH6HTC_hsHUR-Halo

Plasmid

#175303

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HUR (ELAVL1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-human MIZ1 wild type

Plasmid

#74161

Purpose

bacterial expression of MIZ1

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MIZ1 (ZBTB17 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
pJP118

Plasmid

#176494

Purpose

Expresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Insert

Lifeact peptide

Use

Capsaspora owczarzaki expression

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR L1-vas2-Cre-L4

Plasmid

#62301

Purpose

Cre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase under control of vasa promoter and terminator

Tags

HA and SV40 NLS

Expression

Insect

Promoter

vasa

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miR124

Plasmid

#177806

Purpose

The pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-124

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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p458 VRER

Plasmid

#101728

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0508

Plasmid

#91014

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9

Use

CRISPR

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pT3TS-nCas9n-A71

Plasmid

#125644

Purpose

micro-injection of poly-adenylated nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

nCas9n

Use

Rna in vitro transcription vector

Promoter

T3

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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mOPN-PB0

Plasmid

#106449

Purpose

Bacterial expression of murine osteopontin

Depositor

Georg Weber

Article

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

osteopontin (Spp1 Mouse)

Use

Retroviral

Mutation

WT

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRDA_186

Plasmid

#133458

Purpose

U6 promoter expresses customizable Spyo-guide; PGK promoter expresses blasticidin resistance and 2A site provides EGFP

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-GpNLuc_SV40:PuroR

Plasmid

#183037

Purpose

Expresses the FLAG-tagged GpNLuc (Green LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

GpNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBI-GFP-Bub1-S492Y

Plasmid

#16625

Depositor

Bert Vogelstein

Article

Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Insert

BUB1 S492Y (BUB1 Human)

Expression

Mammalian

Mutation

S492Y

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pCEP4 Smad2

Plasmid

#16484

Depositor

Bert Vogelstein

Article

Riggins et al Nat Genet. 1996 Jul . 13(3):347-9.

Insert

Smad2 (SMAD2 Human)

Tags

Double HA

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only