177,711 results
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Plasmid#164513PurposeGreen glucose sensor, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of this plasmid.DepositorInsert(Cyto)iGlucoSnFR
UseAAVMutationSynthetic ConstructPromoterGFAPAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRE:mCherry-p2a- bReaChES
Plasmid#163037PurposeFLiCRE reporter gene for neuronal expressionDepositorInsertmCherry-p2a-bReaChES
UseAAVExpressionMammalianPromoterTREAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSEM238
Plasmid#161515PurposeNeuronal expression of histamine-sensitive chloride channel for negative selectionDepositorInsertpEXP[Psnt-1 | histamine | rpl-3 UTR]
ExpressionWormPromoterPsnt-1Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEG BacMam N term StrepII eGFP 3C
Plasmid#160683PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTagsStrepII eGFP 3CExpressionMammalianPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
JD639
Plasmid#160398PurposeBinary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert35S::vitis miR396-resistant GRF4-GIF1 chimera
UseBinary vectorAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mGreenLantern
Plasmid#161913PurposemGreenLantern fluorescent protein: bacterial expression plasmidDepositorInsertmGreenLantern
Tags6xHisExpressionBacterialPromoteraraBAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK262 (RAD21-mAC Neo)
Plasmid#140538PurposeRAD21 tagging with mAID-CloverDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRAD21 (RAD21 Human)
UseCRISPRExpressionMammalianAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK395 (AAVS1 CMV-OsTIR1(F74A))
Plasmid#159992PurposeExpression of OsTIR1(F74A) in human cellsDepositorInsertCMV-OsTIR1(F74A)
ExpressionMammalianAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus-H2B-mTagBFP2
Plasmid#161790Purpose3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interestDepositorInsertH2B-mTagBFP2
UseLentiviral; BicistronicTagsmTagBFP2ExpressionMammalianMutationI174A in the mTagBFP2 protein according to PLoS O…PromoterUbC and UbC promoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ABI1-dCas9
Plasmid#154474PurposeExpresses ABI1 fused to the N terminus of dCas9DepositorInsertABI1 (ABI1 Mustard Weed)
UseLentiviralExpressionMammalianMutationIncludes ABI1 aa 126-423, D143APromoterCMVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCherry
Plasmid#155222PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
TagsmCherryExpressionMammalianAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-FOXP1
Plasmid#153145PurposeExpression of Flag tagged human FOXP1 in mammalian cellsDepositorAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLEX307-APN-puro
Plasmid#158454PurposeConstitutive lentiviral expression of human APN.DepositorAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
ExpressionBacterialPromoterglnS and pBADAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGαq-LgB97 (GNAQ Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR A757
Plasmid#140829PurposeAIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAIMTOR A757 (ULK1 Human)
TagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…Available sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC
Plasmid#125692PurposeGlobal labeling of inhibitory synapses (green fluorescence)DepositorInsertGephyrin.FingR-eGFP-CCR5TC
UseAAVExpressionMammalianPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
P307-FBP_6
Plasmid#139697PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathwayDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH2B-emiRFP703
Plasmid#136567PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-emiRFP703
TagsemiRFP703ExpressionMammalianPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC-GFP-AT
Plasmid#133306Purpose"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisationDepositorInsertaxe/txe toxin-antitoxin cassette
UseSynthetic BiologyExpressionBacterialAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by