We narrowed to 2,230 results for: TUB

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pN1-RnKIF5C(1-560)-Halo-Flag

Plasmid

#188071

Purpose

Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags

Depositor

Kristen Verhey

Article

Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

Insert

rat KIF5C (Kif5c Rat)

Tags

Flag and Halo

Expression

Mammalian

Mutation

aa1-560

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B G120A

Plasmid

#123073

Purpose

Gateway entry clone encoding human MAP1LC3B G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway Cloning

Mutation

Glycine 120 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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HA-UBE2E1 C131A in pcDNA3.1

Plasmid

#118214

Purpose

Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed cysteine 131 to alanine

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-9A

Plasmid

#114028

Purpose

Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - 9A (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 9A

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/WT M1

Plasmid

#89321

Purpose

expression of M1 C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB2-eGFP-6xHis

Plasmid

#130982

Purpose

Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB2-GFP-6xHis (Mapre2 Mouse)

Tags

eGFP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1deltaC-2xEGFP shRNA resistant

Plasmid

#37925

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 (MAPRE1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion after aa248, TTCCTGT=>TCCCGGT

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M1C448Y

Plasmid

#89319

Purpose

expression of M1 C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, C448Y

Promoter

CMV

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-EGFP MASTL G44S (siRNA resistant)

Plasmid

#191012

Purpose

Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

Depositor

Johanna Ivaska

Article

Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Microtubule-associated serine/threonine kinase like (MASTL Human)

Tags

EGFP

Expression

Mammalian

Mutation

G44S

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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canxTM-mEmerald (cytosolic)

Plasmid

#186962

Purpose

Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

CANX (CANX Human)

Tags

mEmerald

Expression

Mammalian

Mutation

Only express calnexin (482-end) and add ER signal…

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

Plasmid

#129774

Purpose

Marker of microtubule dynamics in C. elegans hypodermis

Depositor

Andrew Chisholm

Article

Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

Inserts

Pcol-19
ebp-2 (ebp-2 Nematode)
unc-54 3'UTR

Use

Gateway Cloning and Synthetic Biology

Tags

Synthetic Intron and mGFP

Expression

Worm

Promoter

Pcol-19

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3B2-GFP

Plasmid

#123109

Purpose

Expresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

Plasmid

#193717

Purpose

Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Human, Synthetic)

Tags

2xmNeongreen-FRB

Expression

Mammalian

Mutation

hsKIF5B(aa1-560), RIGOR: G234A

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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DN1-Gli3 in AINGpCAGGS

Plasmid

#121970

Purpose

medium Gli3 transcriptional activator

Depositor

James Briscoe

Article

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-864)

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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PC4_3spc34h

Plasmid

#70700

Purpose

Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on spc34

Depositor

Stephen Harrison

Article

Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

Insert

DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)

Tags

his-tag on C-terminus of spc34

Expression

Bacterial

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a_DmKHC[1-421]-1xGFP-6xHis

Plasmid

#196972

Purpose

Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

Depositor

Lukas Kapitein

Article

Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

Insert

Kinesin Heavy Chain (Khc Fly)

Tags

6xHis and GFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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actc1b-GcAMP5

Plasmid

#126563

Purpose

Muscle specific expression of the calcium reporter GcAMP5 in zebrafish. Parton lab clone APT

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

GcAMP5

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_A0202

Plasmid

#91003

Purpose

Module A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJAK1.B

Plasmid

#29462

Depositor

Zakaria Hmama

Article

Sun et al Plasmid. 2009 Nov . 62(3):158-65.

Type

Empty backbone

Use

Mycobacteria expression

Tags

M. tuberculosis FbpB signal sequence

Expression

Bacterial

Promoter

Hsp60

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pHLEGM4

Plasmid

#24783

Depositor

Tanya Parish

Article

Carroll et al Appl Environ Microbiol. 2005 Jun . 71(6)

Insert

Ptet-Gfp-ASV

Expression

Bacterial

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only