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Showing: 1141 - 1160 of 1734 results

  1. pAS_4xG1 PylT A41AA C55A sfGFP 150TAG

    Plasmid
    #154770
    Purpose
    plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    150TAG in GFP reporter, G1 PylT with A41AA and C5…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154770-plasmid-map-sequence-id-309867

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  2. pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)

    Plasmid
    #127636
    Purpose
    Encodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)
    Depositor
    Friedrich Simmel
    Article
    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.
    Insert
    mTurquoiuse2-Tat, YPet-PCP
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PCP and RNA binding peptide:…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127636-plasmid-map-sequence-id-251704

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  5. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid
    #225139
    Purpose
    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
    Depositor
    Urartu Seker
    Article
    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.
    Insert
    Ag43 protein fused to silaffin-R5 and sfGFP
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225139-plasmid-map-sequence-id-460505

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  9. pENeCFPCNAL2 pc922

    Plasmid
    #167563
    Purpose
    For construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).
    Depositor
    Cristina Cardoso
    Article
    Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.
    Inserts
    Proliferating Cell Nuclear Antigen (PCNA Human)
    CFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    167563-plasmid-map-sequence-id-403652

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  14. pTone-gata2aECE-nsGFP

    Plasmid
    #132975
    Purpose
    gata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backbone
    Depositor
    Nathan Lawson
    Article
    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d
    Inserts
    nuclear localized sfGFP
    gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
    Use
    Unspecified; Transposon-mediated
    Tags
    6x myc and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    132975-plasmid-map-sequence-id-264810

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  17. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

    Plasmid
    #182395
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-VenusN154
    VNp-LZ-VenusC155
    Use
    Tags
    Vesicle Nucleating peptide (VNp) & Leucine Zi…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    182395-plasmid-map-sequence-id-364922

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  20. Epac-S-H74

    Plasmid
    #170338
    Purpose
    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2011 Apr 29;6(4):e19170. doi:
    Insert
    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170338-plasmid-map-sequence-id-335510

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Showing: 1141 - 1160 of 1734 results