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EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

Plasmid

#120555

Purpose

Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

Depositor

Yu Wang

Article

Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

Insert

Cas9, NES, ERT2 and GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-His RTN3A1

Plasmid

#31087

Depositor

Jimtong Horng

Article

Tang et al J Biol Chem. 2007 Feb 23. 282(8):5888-98

Insert

reticulon 3 (RTN3 Human)

Tags

Myc-His

Expression

Mammalian

Available Since

Aug. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pHLP712

Plasmid

#203741

Purpose

Integration of OsTIR1(F74A)-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Mutation

Codon 74 changed from Phe to Ala (F74A)

Promoter

ACT1 (C. albicans)

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGCP123-GFP_g1

Plasmid

#153518

Purpose

sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pUCmEos3.2:cat

Plasmid

#78100

Purpose

Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.

Depositor

Peng Chen

Article

Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1

Insert

mEos3.2-cat

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-mCherry-TSC2

Plasmid

#192440

Purpose

Encodes mCherry-tagged wild-type TSC2 isoform 5.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2 (TSC2 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMC_mNG2(11)_BSDminus_F2

Plasmid

#184164

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/nV5-DEST-beta catenin

Plasmid

#20140

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pcDNA3.1/nV5-DEST-beta catenin (Ctnnb1 Mouse)

Tags

V5

Expression

Mammalian

Mutation

T3I

Promoter

CMV

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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IRAK1-UTR

Plasmid

#15095

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

IRAK1 3'UTR (IRAK1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 6, 2007

Availability

Academic Institutions and Nonprofits only
gRNA-leu-HYB

Plasmid

#64332

Purpose

leu2 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of leu2 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC22A1

Plasmid

#132034

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A1 (SLC22A1 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pTAL-MajSat-FLAG-NLS-VP64

Plasmid

#69074

Purpose

Expresses TALE for mouse major satellite with VP64 and FLAG under CAG promoter

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

TALE

Expression

Mammalian

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA-DsRed-Express2

Plasmid

#111257

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with DsRed-Express2

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

DsRed-Express2

Expression

Bacterial

Promoter

PpsbA

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCHW111117

Plasmid

#222460

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber mosaic virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-OPTN

Plasmid

#175760

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTN

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-ATG8(414)/GFP-AUT7(414)

Plasmid

#49424

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

Depositor

Daniel Klionsky

Article

Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

ATG8

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCHW110035

Plasmid

#222444

Purpose

Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

LoxP-mCherry-stop

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CD4-myc

Plasmid

#58537

Purpose

Expresses human CD4 with a C-terminal myc tag in mammalian cells

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

CD4 (CD4 Human)

Tags

myc

Expression

Mammalian

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/NS4A

Plasmid

#120826

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS4A ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1

Plasmid

#178603

Purpose

Mammalian expression of myc-tagged MR1

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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