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Plasmid#80603PurposeExpresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus endDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEB1 (MAPRE1 Human)
TagsmCherryExpressionMammalianMutationTruncated C-terminus; EB1 coding sequence ends at…PromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-CRY2-INPP5E
Plasmid#79561Purposeexpression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motifDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertINPP5E (INPP5E Human)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA7
Plasmid#64986PurposeExpresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, E105K, E108A, D16…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hsGFP
Plasmid#64912PurposeEncodes a Fluorescent Reporter for Hydrogen Sulfide in Mammalian CellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthsGFP
Tags6xHisExpressionMammalianMutationChromophore tyrosine of the protein mutated to TA…PromoterCMVAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSA1
Plasmid#52261PurposeBacterial expression of human 6His-tagged SUMO1 and SUMO-E1DepositorTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEYFP(C1)-PP1alpha
Plasmid#44231DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPP1A (PPP1CA Human)
TagsYFPExpressionMammalianPromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BAT-gal
Plasmid#20889DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert7XTCF binding sites, Siamois minimal promoter, NLS-lacz
UseMouse TargetingExpressionMammalianMutation7 multimerized TCF-binding elements cloned upstre…Available sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PiggyBac-TetO-Ascl1-neomycin
Plasmid#176482PurposeTetO-Ascl1 in a PiggyBac vector with neomycin resistanceDepositorAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9-sgRNA-A
Plasmid#149369PurposeExpresses SpCas9 and sgRNA targeting the lenti-CDDR reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
sgRNA-A
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCBh, PGK, and U6Available sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRbeta
Plasmid#136455PurposeMammalian expression plasmid for c-myc-tagged PDGFRBDepositorInsertPDGFRbeta (PDGFRB Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-32a(+)-TGFB1
Plasmid#120361PurposeProduce human recombinant TGFB1 in DE3 cellsDepositorInsertTransforming Growth Factor-β1 (TGFB1 Human, Synthetic)
Tags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC+] (AVA2626)
Plasmid#85444PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA2-gRNA_B
Plasmid#74377PurposegRNA_B to knockout human AMPK alpha 2 using Cas9nDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
UseCRISPRPromoterU6Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK280 (mCherry2-NeoR)
Plasmid#72796PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry2-Bsr
UseCRISPRTagsmCherry2ExpressionMammalianAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRetroX GFP Mps1 wt
Plasmid#63702PurposeInducible expression of GFP-Mps1 fusion in mammalian cellsDepositorAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LV-TRE-WT mouse MyoD-T2A-dsRedExpress2
Plasmid#60624PurposeExpresses WT mouse MyoD and dsRedExpress2 in response to doxycyclineDepositorPublicationAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VV073: 1xCox8 - GCaMP5G in fubi
Plasmid#58509PurposeExpresses the calcium sensor GCaMP5G in the mitochondriaDepositorInsert1xCox8 - GCaMP5G
UseLentiviralExpressionMammalianPromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-N1 human cofilin R21Q
Plasmid#51279Purposeexpresses human cofilin R21Q mutant fused to mRFP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcofilin 1 (CFL1 Human)
TagsmRFPExpressionMammalianMutationR21Q, non-rod-forming mutation that can be used t…PromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMito-LSSmOrange
Plasmid#37135DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLSSmOrange
Tagshuman cytochrome C oxidase subunit VIIIExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-CaMKKbeta
Plasmid#32453DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
TagsFLAG and GFPExpressionMammalianPromoterCMVAvailable sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by