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pISH-Gucy1b2-probe1

Plasmid

#105454

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-PAmCherry1

Plasmid

#189718

Purpose

titratable expression of PAmCherry1 in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

PAmCherry1

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSopBWT

Plasmid

#112512

Purpose

PsopB driven sopB and sigE

Depositor

Olivia Steele-Mortimer

Article

Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

Insert

sopB/sigE operon

Expression

Bacterial

Promoter

PsopB

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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f1H ∂HD (267)

Plasmid

#24426

Depositor

Steven Rosen

Article

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF1 (SULF1 Human)

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

AA 416-726 deleted, 22AA N-terminal truncation

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pJB057

Plasmid

#98911

Purpose

Express Dronpa tagged ZapA in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

ZapA

Tags

Dronpa

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBacPAK p110b WT

Plasmid

#55724

Purpose

His3 GST tagged WT p110 beta

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 beta

Tags

His3 GST

Promoter

polyhedrin promoter

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pMGT1

Plasmid

#109404

Purpose

Tetracycline resistance reporter of ChnR activation

Depositor

Jay Keasling

Article

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

Tetracycline resistance reporter of ChnR activation

Expression

Bacterial

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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IRE-miR21-SunTag-TREAT

Plasmid

#196932

Purpose

SunTag cassette was added in the coding region in the miR21-IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 RNAiR2

Plasmid

#51585

Purpose

expresses RNAi-resistant (RNAiR) myc-tagged mouse Rem2 (long version)

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

silent mutations at shRNA 1 and 2 recognition sit…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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RhoQ

Plasmid

#55695

Purpose

expression of mouse RhoQ

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoQ (Rhoq Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAcrF3

Plasmid

#89246

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

AcrF3

Tags

6xHis-TEV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV 16E6no* I128T

Plasmid

#47706

Purpose

Defective for E6AP binding

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 16 E6no* (E6 HPV)

Tags

FLAG and HA

Expression

Mammalian

Mutation

del first 7 aa, I128T, V42L*

Promoter

CMV

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-Nore1

Plasmid

#1975

Depositor

Joseph Avruch

Article

Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

Insert

Nore1 (Rassf5 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
Lv EF1a MS2-HP1cs 2A Hygro

Plasmid

#102810

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express MS2-HP1cs fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

MS2-HP1cs

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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H2A-Bbd-GFP

Plasmid

#45173

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al J Cell Biol. 2001 Jan 22;152(2):375-84.

Insert

H2AFB1 (H2AFB1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
IZ1181

Plasmid

#21049

Depositor

Tadahide Izumi

Article

Busso et al Oncogene. 2009 Apr 2. 28(13):1616-25.

Insert

human MDM2 (MDM2 Human)

Tags

no tag

Expression

Mammalian

Mutation

no mutation. This is the wild-type human MDM2.

Available Since

July 9, 2009

Availability

Academic Institutions and Nonprofits only
TOPO mKO2

Plasmid

#68442

Purpose

TOPO construct expressing mKusabira-Orange2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mKusabira-Orange2

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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RB793

Plasmid

#199121

Purpose

This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome

Depositor

Richard Bennett

Article

Kakade et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

100 bp barcoding cassette

Expression

Yeast

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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RB794

Plasmid

#199122

Purpose

This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome

Depositor

Richard Bennett

Article

Kakade et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

100 bp barcoding cassette

Expression

Yeast

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Na0.5

Plasmid

#198634

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Na0.5

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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