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pDONR223_CDKN1A_WT

Plasmid

#82201

Purpose

Gateway Donor vector containing CDKN1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDKN1A (CDKN1A Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EBNA1BP2_WT_V5

Plasmid

#82932

Purpose

Gateway Donor vector containing EBNA1BP2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EBNA1BP2 (EBNA1BP2 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMO14

Plasmid

#235736

Purpose

Expression of human VPS34 complex II in mammalian cells

Depositor

Roger Williams

Article

Mercer et al EMBO J. 2021 Jul 15;40(14):e105985. doi:

Inserts

UVRAG (UVRAG Human)
EGFP
BECN1 (BECN1 Human)
PIK3C3 (PIK3C3 Human)
PIK3R4 (PIK3R4 Human)

Tags

2xStrep-tagII-2xTEV-ZZ

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.L858R

Plasmid

#82906

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

L858R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HSP90B1_WT

Plasmid

#82130

Purpose

Gateway Donor vector containing HSP90B1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HSP90B1 (HSP90B1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BMI1_WT

Plasmid

#82194

Purpose

Gateway Donor vector containing BMI1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BMI1 (BMI1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_ID2_WT_V5

Plasmid

#82960

Purpose

Gateway Donor vector containing ID2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ID2 (ID2 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ETS1_WT

Plasmid

#82118

Purpose

Gateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ETS1 (ETS1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHIV-NAT-FLAG-CIP2A FL (sg2R)

Plasmid

#222624

Purpose

Lentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A in mammalian cells

Depositor

Daniel Durocher

Article

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available Since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_BRAF_p.V600E

Plasmid

#81700

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

V600E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

Plasmid

#209084

Purpose

Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
BirA

Expression

Mammalian

Mutation

human codon optimized

Promoter

hSyn

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-iSeroSnFR

Plasmid

#128484

Purpose

Fluorescent reporter for serotonin (mammalian expression, membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Tags

Ig-kappa leader, Myc, PDGFR + enhanced ER export,…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9(MRKCRS)-P2A-EGFP (RAS2821)

Plasmid

#223102

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MRKCRS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MRKCRS)=D1135M, S1136R, G1218K, E1219C, R1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a_His-TEV-EiCsm6

Plasmid

#172487

Purpose

Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

Enterococcus italicus Csm6 (codon optimized)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_CSNK1A1_WT

Plasmid

#82106

Purpose

Gateway Donor vector containing CSNK1A1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Gateway entry vector

Mutation

Q231K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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sgTP53_3

Plasmid

#78164

Purpose

sgTP53

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223_IDH1_p.R132H

Plasmid

#81686

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway entry vector

Mutation

R132H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SETDB1_WT

Plasmid

#82291

Purpose

Gateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SETDB1 (SETDB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMARCB1_WT

Plasmid

#81791

Purpose

Gateway Donor vector containing SMARCB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMARCB1 (SMARCB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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