We narrowed to 5,868 results for: nls

1. pST1374-NLS-flag-linker-Cas9

   Plasmid

   #44758

   Depositor

   Xingxu Huang

   Article

   Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

   Insert

   Human codon optimized Cas9
   

   Use

   CRISPR

   Tags

   32 amino acid linker, Flag, and NLS

   Expression

   Mammalian

   Mutation

   codon-optimized

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

   Plasmid

   #46168

   Purpose

   Codon optimized Cas9_SV40 NLS with intron

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   codon optimized Cas9_SV40 NLS with intron
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   intron inserted at position 3113-3163, and SV40 N…

   Promoter

   eef-1A.1 (eft-3)

   Available since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-TRE3G-FNLS-PGK-Puro

   Plasmid

   #110847

   Purpose

   Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   TRE3G

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. YFP NLS Beta-Actin S14C

   Plasmid

   #60614

   Purpose

   Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

   Depositor

   Primal de Lanerolle

   Article

   Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

   Insert

   Beta-actin (ACTB Human)
   

   Use

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   S14C

   Promoter

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207376

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR

   Plasmid

   #154003

   Purpose

   Vector expressed CasRx and sgRNA_LACZ for AAV package

   Depositor

   Hui Yang

   Article

   He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

   Insert

   CasRx
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

   Plasmid

   #86827

   Purpose

   Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   FLAG-NLS-MS2-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

8. AAVS1-NeoR-CAG-EGFPnls-WPRE

   Plasmid

   #191692

   Purpose

   For knocking pCAG-EGFPnls into AAVS1 locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   AAVS1 homology arms and pCAG-EGFPnls
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

   Plasmid

   #100941

   Purpose

   Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
   

   Use

   Tags

   1xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-EFS-PCP-3XNeon-nls

    Plasmid

    #75386

    Purpose

    PCP-3XNeon-nls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    PCP
    

    Use

    Lentiviral

    Tags

    3XmNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. BC1177-pHR-SFFV-GFP(1-10)-NLS

    Plasmid

    #164039

    Purpose

    Expression of GFP1-10 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    GFP(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDonor-tBFP-NLS-Neo (Universal)

    Plasmid

    #80767

    Purpose

    Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Insert

    PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-Synapsin-mCherry-3XNLS-WPRE

    Plasmid

    #159951

    Purpose

    nucleus marker

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mCherry-3XNLS
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. A3Bi-ctd-Cas9n-UGI-NLS

    Plasmid

    #109426

    Purpose

    Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUBC-SV40NLS-Pfv-Sapphire-PURO

    Plasmid

    #203654

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-eSpCas9-plus-NLS-6xHis

    Plasmid

    #207380

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, R1060A, amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LLP453 pEf1a-NLS-aGCN4-mCherry

    Plasmid

    #100945

    Purpose

    mCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    scFvGCN4, sfGFP, GB1, mCherry
    

    Use

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCVL SFFV-EF1s HA.NLS.Sce(opt)

    Plasmid

    #31479

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV/Ef1s HA NLS I-SceI
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    "opt" indicates the coding sequence has…

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80063

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Sox10 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-TetOne-zeo_3xHA-TurboID-NLS

    Plasmid

    #200022

    Purpose

    For lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoter

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    3xHA-TurboID-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3Gs

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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