We narrowed to 7,456 results for: Lif
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Plasmid#158765PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-RFP-IRES-CD
Plasmid#126678PurposeDoxycycline inducible expression of CD along with reporter RFPDepositorInsertRFP-IRES-CD (FCY1 Budding Yeast)
UseTagsExpressionMutationPromotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 R443A
Plasmid#162652PurposeExpresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acid R443 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-tdTomato-ChIEF
Plasmid#172370PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInserttdTomato + ChIEF
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109HgAmp30E
Plasmid#123149PurposeMercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerT, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
Plasmid#125225PurposeExpresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cellsDepositorInsertgLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2ADW-lox-mCherry-hyBRET-ERK-NLS
Plasmid#170290PurposeFor generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.DepositorInsertlox-mCherry-lox-hyBRET-ERK-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T VPS4B, residues 1-86
Plasmid#180639PurposeBacterial expression for VPS4B MIT domain, residues 1-86. Has N-terminal GST tag. Internal ID: WISP04-155.DepositorInsertVPS4B (VPS4B Human)
UseTagsGSTExpressionBacterialMutationResidues 1-86PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry_SNAP_SNAPV_PACT_Dead
Plasmid#173073PurposeDead SNAP tag at the centrosomeDepositorInsertmCherry-SNAP-SNAP-Varied-PACT
UseTagsmCherry-SNAP-SNAP-VariedExpressionMammalianMutationAA144 in both SNAP tags changed from a cysteine t…PromoterCMV IE94Available sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
All-in-one split-ADAR2
Plasmid#170136PurposeAAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signalDepositorInsertADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alaninePromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-POLD3
Plasmid#183197PurposeEntry cloneDepositorInsertCas9-POLD3
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mcherry-p62 Double Mutation/Silent
Plasmid#187982PurposeK7A/D69A p62 double mutant (PB1AA). Internal reference: SMC532DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationD7A, D69A, silent mutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro
Plasmid#129537PurposePiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.DepositorInserttetR-3xFlag-HA-2A-Puro
UseSynthetic BiologyTagsExpressionMutationPromoterhPGK promoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH3456
Plasmid#179581PurposePnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 BFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagsBFPExpressionWormMutationPromoterPnmr-1Available sinceMarch 2, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCL1316
Plasmid#217958PurposeExpression of human H4 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H4 (H4C1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2pA2-drl:APEX2-mCherry
Plasmid#188945PurposePlasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoterDepositorInsertdrl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA
UseTol2 destination vector (attr4-r3)TagsExpressionMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ER-BSCaM2
Plasmid#64855PurposeEndoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-BSCaM2
UseTagsCFP, N-terminal 27 amino acids from CYP450, and Y…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-EYFP
Plasmid#172383PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertEYFP
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-mCitrine
Plasmid#172384PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertmCitrine
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-EGFP-ChIEF
Plasmid#172371PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertEGFP + ChIEF
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.EGFP-FlpO
Plasmid#172379PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized EGFP + FlpO
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-owlTRIMCyp-FOS
Plasmid#79067PurposeExpress owl monkey TRIMCyp in mammalan cellsDepositorInsertTRIM5Cyp (owl monkey)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Nxph4-HA
Plasmid#128102PurposeExpress HA tagged Nxph4DepositorInsertNxph4 (Nxph4 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4
Plasmid#125230PurposeFor generating SPARK NanoLuc-oβ2AR transmembrane component lentivirusDepositorInsertgLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4
UseLentiviralTagsHA,FlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP
Plasmid#185687PurposeLow-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).DepositorInsertERmembrane-GST-2xHaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-SypRFP
Plasmid#172381PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertSynaptophysin1-RFP fusion protein
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-ACG site)
Plasmid#170155PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editingDepositorInsertBoxB-RAB7A(ACG)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFPnls barcode-206-SV40 polyA
Plasmid#190875PurposeFor barcoded retrograde labelingDepositorInsertEGFPnls-barcode206
UseAAVTagsExpressionMutationWTPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-RFC5
Plasmid#183200PurposeEntry cloneDepositorInsertCas9-RFC5
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only