We narrowed to 27,658 results for: STI
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p663-UBC-miniTurbo-V5-CSNK1G3_IDG-K
Plasmid#214460PurposeGateway destination clone of CSNK1G3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1G3 (CSNK1G3 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianPromoterUbiquitinAvailable SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK1G1_IDG-K
Plasmid#214458PurposeGateway destination clone of CSNK1G1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1G1 (CSNK1G1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianPromoterUbiquitinAvailable SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015
Plasmid#202024PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
ExpressionBacterialMutationGvpA T6A-L40AAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
XLone-GvpB
Plasmid#173798PurposeMammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)DepositorInsertGvpB
TagsmCherry (K187N -please see depositor comments)ExpressionMammalianPromoterTRE3G promoterAvailable SinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pThuAsol
Plasmid#171675PurposepThuAsol expression in E. coliDepositorInsertSUMO-TEV-ThuAsol
TagsHis-tag and SUMO-tagExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FoxFTVC+bpFOG_MRAS-G22V
Plasmid#107521PurposeConstitutive activation of FGF/MAPK pathway by M-Ras-G22V mutantDepositorInsertM-Ras protein
Mutationchanged Glycine 22 to ValinePromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGH50
Plasmid#107254PurposeVipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.DepositorInsertvipp1-gfp-fkbp12
UseSuicide vector for destined for double homologous…TagsGFP and FKBP12ExpressionBacterialPromoternativeAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/C448Y with 5'UTR
Plasmid#89318Purposeexpression of C448Y spastin M1 and M87 in mammalian cellsDepositorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87C448Y
Plasmid#89329PurposeInducible expression of M87 C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
ExpressionMammalianMutation1-460 bp deleted, C448YPromoterTet-responsive P tightAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP C448Y with 5'UTR
Plasmid#89325PurposeInducible expression of C448Y spastin M1 and M87 in mammalian cellsDepositorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1C448Y
Plasmid#89326PurposeInducible expression of M1C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
ExpressionMammalianMutation1-194 bp of 5'UTR deleted, C448YPromoterTet-responsive P tightAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)
Plasmid#185913PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AC EJ7-GFP
Plasmid#113617PurposeReporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFPDepositorInsertEJ7-GFP variant of EGFP
ExpressionMammalianAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti6-SLC7A11/xCT-V5
Plasmid#170427PurposeEepresses SLC7A11 (also named xCT) in mammalian cellsDepositorInsertsolute carrier family 7 member 11 (SLC7A11 Human)
UseLentiviralTagsV5ExpressionMammalianAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)
Plasmid#185914PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…PromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
hprtSAGFP
Plasmid#41594PurposeIn vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequencesDepositorInsertSA-GFP reporter
UseMouse Targeting; Dsb repair reporter substrateExpressionMammalianAvailable SinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRosetta_v2
Plasmid#136477PurposeLentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycinDepositorInsertHygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
UseLentiviralPromoterPGKAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only