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ATP1A1_G6_T804N_MTOR_E2419K_Dual_pegRNA

Plasmid

#178110

Purpose

Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-E2419K pegRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G6 T804N pegRNA + MTOR-E2419K pegRNA

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCK-D390TN(UniProt)-FLAG

Plasmid

#164693

Purpose

Express N-terminally truncated DroshaTN-FLAG in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

N-terminally truncated transdominant negative Drosha (DROSHA Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf222

Plasmid

#12910

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf222

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf264

Plasmid

#12952

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf264

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf194

Plasmid

#12882

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf194

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pCI-Neo-gp78/2853

Plasmid

#11435

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78 (AMFR Human)

Expression

Mammalian

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GATA1 K137R

Plasmid

#85694

Purpose

Mammalian expression of mouse GATA1 with K137R mutation

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Mutation

K137R (SUMO-1 attachment site)

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
p6595 MSCV-IP-N-HA-GPRC5B

Plasmid

#34894

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

GPRC5B (GPRC5B Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
GFP-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#79676

Purpose

mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L182A/D183A/I184A/G185A catalytic mutant

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
p6900 PHAGE-P CMVT N-HA-hE6AP III C840A

Plasmid

#37606

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP III C840A (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C840A

Promoter

CMV tet regulatable

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
Venus-G protein Gamma 5

Plasmid

#42200

Depositor

Narasimhan Gautam

Article

Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

Insert

Gamma 5 (Gng5 Rat)

Tags

Venus(1-155)

Expression

Mammalian

Mutation

codon-optimized

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-CUL1 L756A/I757A

Plasmid

#19938

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 L756A/I757A (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

Mutation of two adjacent hydrophobic residues, L…

Available Since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
pRSETa-Amrose v1

Plasmid

#79537

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 1

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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SNX3-PX (1-162)

Plasmid

#119084

Purpose

Bacterial expression of human phox homology (PX) domain, SNX3-PX (1-162)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX3-PX (1-162) (SNX3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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XE223 human myc-Chibby-pCS2+MT

Plasmid

#16825

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

myc-Chibby (CBY1 Human)

Use

Xenopus expression

Tags

6-myc

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pPKm- 226

Plasmid

#90497

Purpose

pcDNA3 - CMVmin - PIF3 - VPR expresses PIF3 and VPR transactivator domain , under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

PIF3-VP64

Expression

Mammalian

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1-SLC35B2-FLAG

Plasmid

#154863

Purpose

Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

ctcctggtgcagtacttc => ttattagtccaatatttt

Promoter

CMV

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPKm-293

Plasmid

#105817

Purpose

pcDNA - TET DBD - MTAD. Expresses TET DNA binding domain fused to MTAD

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

TET DBD and MTAD

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pPN461

Plasmid

#137877

Purpose

PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

POGZ (POGZ Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR 7SK-miR-30 L5-L4

Plasmid

#62119

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only