159,557 results

1. PA-mCherry1-H2B-C-10

   Plasmid

   #57181

   Purpose

   Localization: Histones/Nucleus, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEZYflag

   Plasmid

   #18700

   Purpose

   pFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pB PUb-integrase

   Plasmid

   #183966

   Purpose

   piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   PhiC31 integrase
   

   Use

   Piggybac insect transgenesis destination vector

   Tags

   Expression

   Mutation

   Promoter

   Aedes aegypti Polyubiquitin

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1 3xF-dyskerin

   Plasmid

   #126870

   Purpose

   Expresses 3xFlag dsykerin

   Depositor

   Kathleen Collins

   Article

   Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

   Insert

   Dyskerin
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Cavb3

   Plasmid

   #26574

   Depositor

   Diane Lipscombe

   Insert

   calcium channel beta subunit-III (Cacnb3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. G13 sensor (Ga13_2)

   Plasmid

   #112930

   Purpose

   A FRET sensor for G13 activation, encoded on a single plasmid.

   Depositor

   Dorus Gadella

   Article

   Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

   Insert

   G13 sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

   Plasmid

   #26966

   Purpose

   AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Article

   Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   eNpHR 3.0
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   ER export at 3' end of gene. Trafficking sig…

   Promoter

   EF1alpha

   Available since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONRPEX18Tp-SceI-pheS

   Plasmid

   #111608

   Purpose

   Gateway compatible allelic exchange vector for Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Type

   Empty backbone

   Use

   Allelic exchange in burkholderia sp.

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. xCas9(3.7)-ABE(7.10)

   Plasmid

   #108382

   Purpose

   Mammalian expression vector for xCas9(3.7)-ABE

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.7)-ABE(7.10)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLARRY-EGFP

    Plasmid

    #140025

    Purpose

    Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

    Depositor

    Fernando Camargo

    Article

    Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMCL-HA-MAPKK1-11A55B [S218E/S222D]

    Plasmid

    #40809

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S218E, S222D

    Promoter

    CMV

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pTcI38-Wasabi

    Plasmid

    #86117

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI38
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Promoter

    pR/pL and pR/pL, LacI

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mAIRN HO

    Plasmid

    #207411

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mAIRN CD

    Plasmid

    #207412

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. IFI16-FL-(1-729)

    Plasmid

    #35051

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16 (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pEGFP_sfCherry2(11)_H2B

    Plasmid

    #82605

    Purpose

    Expresses sfCherry2(11) tagged H2B in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_H2B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Gly12 to Ala in sfCherry11+

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCGN-ELK-1

    Plasmid

    #27156

    Purpose

    For mammalian expression of human Elk-1 with an HA tag

    Depositor

    Ron Prywes

    Article

    Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

    Insert

    ELK1 (ELK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG_smFP FLAG

    Plasmid

    #59756

    Purpose

    encodes multiple copies of an epitope tag FLAG

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-Clover

    Plasmid

    #163366

    Purpose

    Backbone for generating N-terminal or C-terminal clover constructs

    Depositor

    Violaine Sée

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mEos3.2-N1

    Plasmid

    #54525

    Purpose

    Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Tao Xu, Michael Davidson

    Article

    Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

    Type

    Empty backbone

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge