We narrowed to 17,223 results for: EMB
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Plasmid#227313PurposeDonor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)DepositorInsertCLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSems leader-ALFA-mXFP-TpoR
Plasmid#222945PurposeExpression of TPOR with ALFAtag and monomeric XFP in mammalian cellsDepositorInsertThrombopoietin receptor (26-635) (MPL Human)
TagsALFAtag, Ig k-chain leader sequence, and mXFPExpressionMammalianMutationmXFP: tryptophan 66 to phenylalanine, TPOR: Signa…PromoterCMVAvailable SinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTagGFP2-N SIN1 NCD
Plasmid#124927PurposeFor expression of NCD (a.a. 1-139) of SIN1-GFPDepositorInsertMAPKAP1 (MAPKAP1 Human)
TagsTagGFP2ExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…Available SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME Actin Vhh Halo Tag (JDW 1223)
Plasmid#224498PurposeGateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)DepositorInsertActin-Vhh-Halo-Tag
UseGateway cloningAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) WT
Plasmid#108916PurposeExpress WT Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEX-YFP-STIM1-deltaK
Plasmid#18861DepositorInsertSTIM1 (STIM1 Human)
TagsYFPExpressionMammalianMutationStop codon inserted at amino acid postion 671 of …Available SinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
LRRC8b-His-bio
Plasmid#53362PurposeExpresses full-length Leucine rich repeat containing 8 family, member B ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertLRRC8b (LRRC8B Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-ultraID-LMNB1
Plasmid#207775PurposeDonor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorInsertLMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac SpyTag-β2AR-SpyTag
Plasmid#135620PurposeExpression of SpyTag-B2AR in insect cellDepositorInsertBeta 2 adrenergic receptor (ADRB2 Human)
TagsHA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tagExpressionInsectMutationDeleted 1-24 aa, 366-413 aaAvailable SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-MAPRE1
Plasmid#207794PurposeDonor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793DepositorInsertMAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
nAChR beta2 CFP
Plasmid#15106DepositorAvailable SinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mScarlet-ACTB
Plasmid#207754PurposeDonor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorInsertACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEX-CFP-STIM1-deltaK
Plasmid#18860DepositorInsertSTIM1 (STIM1 Human)
TagsCFPExpressionMammalianMutationStop codon at amino acid position 671 of STIM1. C…Available SinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Dcst2-3xHA
Plasmid#183543PurposeExpression vector of mouse DC-STAMP domain containing 2 (Dcst2) tagged with 3xHA at C-terminus.DepositorAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-bio-His
Plasmid#51809PurposeExpresses full-length Lysosome-associated membrane glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertLAMP1 (LAMP1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-LRRK2 Armadillo 1-159
Plasmid#186016Purpose2xHis-LRRK2 Armadillo 1-159DepositorInsert2xHis-LRRK2 Armadillo 1-159 (LRRK2 Human)
ExpressionBacterialMutationSee depositor's comments below.Available SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-LRRK2 Armadillo 350-550
Plasmid#186018Purpose2xHis-LRRK2 Armadillo 350-550DepositorInsert2xHis-LRRK2 Armadillo 350-550 (LRRK2 Human)
ExpressionBacterialAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-4E-pEGFP-C1
Plasmid#166744PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, Bik with mutation in BH3 domain to disrupt binding anti-apoptotic proteinsDepositorInsertBik-4E (BIK Human)
TagsVenusExpressionMammalianMutation4 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Chicken ArcLight Q175
Plasmid#53566PurposeGenetically encoded voltage sensor ArcLight with improved kineticsDepositorInsertChicken ArcLight-Q175
ExpressionMammalianMutationGg-VSP contains an R153Q mutation and an amino ac…PromoterCMVAvailable SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-FKBP-INPP5ED477N
Plasmid#202738Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only