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pMXs-OLIG2

Plasmid

#32933

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

OLIG2 (OLIG2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pFS181

Plasmid

#12536

Depositor

Nick Rhind

Article

Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Insert

human equilibrating nucleoside transporter 1 (SLC29A1 Human)

Expression

Yeast

Available Since

Sept. 5, 2006

Availability

Academic Institutions and Nonprofits only

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TFORF0233

Plasmid

#142567

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MNX1 (MNX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Annexin-12

Plasmid

#55276

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Annexin (A4) (ANXA4 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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GPR142-Tango

Plasmid

#66319

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR142 (GPR142 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
TFORF2523

Plasmid

#142523

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NRF1 (NRF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_RPA2:1-270

Plasmid

#211013

Purpose

Mammalian expression of full-length human RPA2. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RPA2:M1-E270 (RPA2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C3-hYAP2

Plasmid

#17844

Depositor

Marius Sudol

Article

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Insert

YAP (YAP1 Human)

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-ARAP3

Plasmid

#39484

Depositor

Sonja Vermeren

Article

Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

Insert

ARAP3 (ARAP3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
TFORF1874

Plasmid

#143329

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

BBX (BBX Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-GATA6

Plasmid

#101418

Purpose

Donor Vector containing GATA6 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

GATA6 (GATA6 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6-G1-ATP6-Myc-FLAG (puromycin)

Plasmid

#86851

Purpose

mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP6 (ATP6 Human)

Tags

ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

Expression

Mammalian

Mutation

codon corrected

Promoter

CMV

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)

Plasmid

#185447

Purpose

Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cells

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Inserts

TRF1 (TERF1 Human)
POT1 (POT1 Human)
TPP1 (TPP1 Human)
TIN2 (TINF2 Human)

Tags

MBP, YBBR, and ZZ

Expression

Insect

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF2951

Plasmid

#144427

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TWIST2 (TWIST2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCS-H2B-EGFP

Plasmid

#53744

Purpose

Contains human Histone H2B fused to EGFP in the pCS2+ expression vector

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

EGFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

June 27, 2014

Availability

Academic Institutions and Nonprofits only

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TFORF1968

Plasmid

#143561

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

YY1 (YY1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-ARNTL-shRNA1

Plasmid

#166913

Purpose

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNTL shRNA (BMAL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJAM1.21_PB-UGCG-PGK-Blast

Plasmid

#205566

Purpose

Express human UGCG

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

UGCG (UGCG Human)

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab13

Plasmid

#110500

Purpose

Fluorescent Rab13

Depositor

Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

Rab13 (RAB13 Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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FPR1-Tango

Plasmid

#66283

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

FPR1 (FPR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only