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GIT1-Flag/pBK

Plasmid

#197313

Purpose

Plasmid expressing an antigen targeting GIT1

Depositor

Eunjoon Kim

Insert

GIT1 (Git1 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC(elavl3)-CaMPARI2

Plasmid

#137185

Purpose

pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA

Use

Tol2 plasmid for zebrafish

Tags

FLAG, HA, myc

Promoter

HuC(elavl3)

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-SYN_mEGFP-CREB

Plasmid

#137006

Purpose

AAV backbone, mEGFP-CREB donor for G-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

AAV

Tags

mEGFP

Expression

Mammalian

Promoter

hSynapsin

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-SYN_mCherry-KIX-mCherry

Plasmid

#137007

Purpose

AAV backbone, mCherry-KIX acceptor for G-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

KIX domain (Crebbp Rat)

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

hSynapsin

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVTREGCaMP6f

Plasmid

#97410

Purpose

Amplified expression of GCaMP6f in combination with TET driver

Depositor

Tetsuo Yamamori

Article

Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

TRE3

Available Since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pmRFP-LC3

Plasmid

#21075

Purpose

Mammalian expression of rat LC3 fused to mRFP

Depositor

Tamotsu Yoshimori

Article

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

mRFP

Expression

Mammalian

Mutation

First ATG is not included.

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 F-tractin-EGFP

Plasmid

#58473

Purpose

Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

F-tractin (Itpka Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti sfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164478

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-GFP-LC3-RFP

Plasmid

#84573

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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RIP-Timer (DM#285)

Plasmid

#15109

Depositor

Douglas Melton

Article

Dor et al Nature. 2004 May 6. 429(6987):41-6.

Insert

insulin II promoter (Ins2 Rat)

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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psfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164477

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 IP3R3 WT

Plasmid

#236453

Purpose

transient overexpression of IP3R3 in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

IP3R3 (Itpr3 Rat)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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CMV mRuby3-Synapsin1a

Plasmid

#187896

Purpose

For labeling pre-synaptic terminals

Depositor

Timothy Ryan

Insert

Synapsin1a

Tags

mRuby3

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-OMP25

Plasmid

#141149

Purpose

Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat, Budding Yeast)

Tags

SNAP tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mRuby2-Lysosomes-20

Plasmid

#55902

Purpose

Localization: Lysosome Membrane, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LAMP1

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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CMV mScarlet-LC3B

Plasmid

#200431

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-LC3

Plasmid

#184900

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6f

Plasmid

#40755

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6f

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pMMTV neu NT

Plasmid

#1823

Depositor

Philip Leder

Article

Muller et al Cell 1988 Jul 1;54(1):105-15.

Insert

neu NT (Erbb2 Rat)

Expression

Mammalian

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only