We narrowed to 191 results for: LOV2

1. pLuc-OptoJNKi-lsm

   Plasmid

   #89746

   Purpose

   Expression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi lit-state mutant
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   I539E mutation of AsLov2Jalpha

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLuc-OptoJNKi-dsm

   Plasmid

   #89745

   Purpose

   Expression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi dark-state mutant
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   C450A mutation of AsLov2Jalpha

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLuc-Optop38i-dsm

   Plasmid

   #89748

   Purpose

   Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   Optop38i dark-state mutant
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   C450A mutation of AsLov2Jalpha

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFT4.2

   Plasmid

   #208807

   Purpose

   Expresses the fluorescent protein phiLOV2.1

   Depositor

   François-Xavier Campbell-Valois

   Article

   Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

   Insert

   phiLOV2.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET-MagLOV

   Plasmid

   #219957

   Purpose

   Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

   Depositor

   Andrew York

   Article

   Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

   Insert

   MagLOV
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   AsLOV2 C450P L496V Q513K G528K D540M

   Promoter

   T7

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHIS8-3_DL121

   Plasmid

   #171953

   Purpose

   Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

   Depositor

   Kimberly Reynolds

   Article

   McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

   Insert

   Fusion of ecDHFR and asLOV2 at position 121 of DHFR
   

   Use

   Tags

   8-His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYCDuet_DL121_WTTS

   Plasmid

   #171954

   Purpose

   Selection vector for chimeric fusion of ecDHFR and asLOV2.

   Depositor

   Kimberly Reynolds

   Article

   McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

   Inserts

   Fusion of ecDHFR and asLOV2 at position 121 of DHFR
   Thymidylate Synthase
   

   Use

   Tags

   8-His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Actin-OptoNB

   Plasmid

   #159595

   Purpose

   Opto-Nanobody

   Depositor

   Jose Avalos, Jared Toettcher

   Article

   Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

   Insert

   ActinNB with AsLOV2 at loop 5 with 62-666
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQP-iRIS

   Plasmid

   #102584

   Purpose

   Regulation of protein localization (near-infrared-blue-light-inducible shuttle)

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

   Insert

   BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. LaM8-AK74-irFP

    Plasmid

    #159592

    Purpose

    Opto-Nanobody

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    LaM8 with AsLOV2 at AK74
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCri_L830-antiSH2-OptoMB-V416L

    Plasmid

    #159936

    Purpose

    Opto-MB bacterial expression

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

    Insert

    anti-SH2 MB with SS58 insert
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AsLOV2 V416L

    Promoter

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCri_L889-antiSH2-OptoMB-Triple

    Plasmid

    #159937

    Purpose

    Opto-MB bacterial expression

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

    Insert

    anti-SH2 MB with SS58 insert
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AsLOV2 V416I_G528A_N538E

    Promoter

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. LaM8-AK74

    Plasmid

    #159594

    Purpose

    Opto-Nanobody

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    LaM8 with AsLOV2 at AK74
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LaM8-GG15-irFP

    Plasmid

    #159591

    Purpose

    Opto-Nanobody

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    LaM8 with AsLOV2 at GG15
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQP-CiRISB

    Plasmid

    #138978

    Purpose

    Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LaM8-GG15

    Plasmid

    #159593

    Purpose

    Opto-Nanobody

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    LaM8 with AsLOV2 at GG15
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTrc_AraC-I113-LOV

    Plasmid

    #206804

    Purpose

    Expresses AraC-I113-LOV from a Trc promoter. For use in E. coli

    Depositor

    Dominik Niopek

    Article

    Mathony et al Adv Sci (Weinh). 2023 Oct;10(28):e230349

    Insert

    AraC-AsLOV2 fusion
    

    Use

    Synthetic Biology

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTrc_AraC-S170-LOV

    Plasmid

    #206805

    Purpose

    Expresses AraC-S170-LOV from a Trc promoter. For use in E. coli

    Depositor

    Dominik Niopek

    Article

    Mathony et al Adv Sci (Weinh). 2023 Oct;10(28):e230349

    Insert

    AraC-AsLOV2 fusion
    

    Use

    Synthetic Biology

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQP-NiRISB

    Plasmid

    #138977

    Purpose

    Light-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQP-iRISB

    Plasmid

    #138979

    Purpose

    Light-controlled tri-directional targeting of endogenous proteins

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)

    Plasmid

    #203907

    Purpose

    Encodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    Gal4LOV(SK22)-VP64-IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

    Plasmid

    #203911

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C2]-VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

    Plasmid

    #203910

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C1]-VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PAC_E83_LOV_eGFP

    Plasmid

    #231574

    Purpose

    This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

    Depositor

    Dominik Niopek

    Article

    Wolf et al bioRxiv 2024.12.04.626757

    Insert

    PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDN77

    Plasmid

    #61347

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS2 and NES IkBa

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDN34

    Plasmid

    #61343

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS2 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDN80

    Plasmid

    #61349

    Purpose

    LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    AsLOV2 wt and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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28. pDB22

    Plasmid

    #61342

    Purpose

    Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-cMyc1A NLS and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDN42

    Plasmid

    #61345

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS10 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDN41

    Plasmid

    #61344

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS9 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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