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Showing: 201 - 240 of 482 results

  1. pEMS1424

    Plasmid
    #29220
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    Ple155-EGFP-NLS
    Use
    High copy number homologous recombination plasmid…
    Expression
    Mammalian
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available Since
    Nov. 16, 2011
    Availability
    Academic Institutions and Nonprofits only
    29220-plasmid-map-sequence-id-13635

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  7. pSAH031

    Plasmid
    #90330
    Purpose
    Promoterless iLOV gene for recombineering, flanked by BsmbI restriction sites.
    Depositor
    David Savage
    Insert
    iLOV
    Tags
    None
    Promoter
    None
    Available Since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    90330-plasmid-map-sequence-id-189687

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  12. pJM105: pTac RFP on pCM66T

    Plasmid
    #74746
    Purpose
    pCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    pTac
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74746-plasmid-map-sequence-id-146957

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  16. pDEST-N2H-F1-empty vector

    Plasmid
    #125551
    Purpose
    N2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125551-plasmid-map-sequence-id-252311

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  29. yPet_cp229-Binder

    Plasmid
    #179137
    Purpose
    YPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).
    Depositor
    Klaus Hahn
    Article
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Insert
    SspB
    Tags
    yPet_cp229
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179137-plasmid-map-sequence-id-353407

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  35. pAC-aZEA

    Plasmid
    #53286
    Purpose
    Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    lcyE (LUT2 Mustard Weed)
    Use
    Low copy number bacterial cloning vector
    Promoter
    lac
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53286-plasmid-map-sequence-id-83099

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  37. pRaGE Pyl TAG GFP Y35TAG

    Plasmid
    #160041
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5
    Inserts
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    deGFP
    Use
    Synthetic Biology
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed tyrosine 35 to TAG stop-codon (Site numbe…
    Promoter
    P70b promoter and T500 terminator, Vibrio natrieg…
    Available Since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160041-plasmid-map-sequence-id-332393

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Showing: 201 - 240 of 482 results