We narrowed to 733 results for: Tcta
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Plasmid#68437PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.DepositorInsertTOP1 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U1Available SinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV/3' Box_(GLuc)_TOP2
Plasmid#68435PurposeTransient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertTOP2 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable SinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR-puro-Exo84
Plasmid#31116DepositorInsertExo84 shRNA (EXOC8 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable SinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[Spinach2]
Plasmid#68430PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the "Spinach2" aptamer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-crRNA4n96
Plasmid#123640PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertcrRNA scaffold 4n96
UseTagsExpressionMammalianMutationPromoterAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_TOP1
Plasmid#68423PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertTOP1 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_VEGFA
Plasmid#183327PurposeAll-in-One CRISPRko system with a guide RNA that targets VEGFA geneDepositorInsertVEGFA
UseLentiviralTagsExpressionMutationPromoterAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.shNkx2-1
Plasmid#32400DepositorInsertshRNA targetting Nkx2-1 (Nkx2-1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgSetd2#1/Cre
Plasmid#89649PurposeExpresses a Setd2-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Setd2 (Setd2 Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJZC593
Plasmid#62319PurposesgRNA with MS2-PP7 for yeast cellsDepositorInsertsgRNA + MS2-PP7 RNA binding module
UseTagsExpressionYeastMutationPromoterSNR52Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR1
Plasmid#183289PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR1 geneDepositorInsertFGFR1
UseLentiviralTagsExpressionMutationPromoterAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pADH118-2
Plasmid#90984PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. albicans LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNAmreB
Plasmid#149653Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AasgRNA3.8.3
Plasmid#121959PurposeMammalian expression, Transcription regulation, gRNA scaffoldDepositorInsertAaCas12b single chimeric gRNA, MS2 hairpin inserted
UseTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pADH143-2
Plasmid#90990PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. maltosa LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH100-2
Plasmid#90981PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[GFPApt]
Plasmid#68428PurposeTransient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the GFP aptamer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xK-T]
Plasmid#68429PurposeTransient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing three kink-turns
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTBL3428 MTK234-LacO-BtoApegRNA
Plasmid#226669PurposeMTK234 part containing lac-inducible variant of the hU6 promoter driving a B to A pegRNADepositorInserthU6-LacOS-BtoApegRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-LacOSAvailable SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3346 PB-nicking guide-blast
Plasmid#226665PurposePiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidinDepositorInsertprotective nicking sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-non-targeting-control -GFP
Plasmid#221844PurposeTol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoterDepositorInsertsEGFP
non-targeting control sgRNA- GCACTGCTACGATCTACACC
UseCRISPR; Tol2 transposon optimised for chick expre…TagsExpressionMammalianMutationPromoterGACG and U6.3 chickAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Merry Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210743PurposeCoding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 3DepositorInsertsSaSp Cas9
Merry sgRNA GFP Target 3
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Merry Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210744PurposeCoding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 4DepositorInsertsSaSp Cas9
Merry sgRNA GFP Target 4
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Merry Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210741PurposeCoding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 1DepositorInsertsSaSp Cas9
Merry sgRNA GFP Target 1
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Merry Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210742PurposeCoding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 2DepositorInsertsSaSp Cas9
Merry sgRNA GFP Target 2
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_ST-sgRNA
Plasmid#188705PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting lociDepositorInsertST sgRNAs
UseLentiviralTagsExpressionMammalianMutationPromoterhUbCAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC5
Plasmid#191860PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT-GFP-rG2
Plasmid#188968PurposeIPTG inducible GFP with sgRNADepositorInsertsGFP
sgRNA: agtccatgtaatcagcgtctactagt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrcAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_DRB
Plasmid#164992PurposeExpression of gRNA targeting HLA-DRB locus, including DRB1*01:02:01DepositorInsertgRNA against HLA-DRB1*01:02:01
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_HDAC1
Plasmid#183297PurposeAll-in-One CRISPRko system with a guide RNA that targets HDAC1 geneDepositorInsertHDAC1
UseLentiviralTagsExpressionMutationPromoterAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_CDK4
Plasmid#183275PurposeAll-in-One CRISPRko system with a guide RNA that targets CDK4 geneDepositorInsertCDK4
UseLentiviralTagsExpressionMutationPromoterAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_sgRNA_Ago1_5
Plasmid#172470PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1DepositorInsertsgAgo1
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0526-sgRNArodA
Plasmid#149658Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0526Available SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNArodA
Plasmid#149569PurposerodA gRNA expression in B. burgdorferiDepositorInsertsgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNArodA
Plasmid#149655Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAmreB
Plasmid#149567PurposemreB gRNA expression in B. burgdorferiDepositorInsertsgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
LEP-shTAF4.3818
Plasmid#105564Purposeretrovirally express TAF4 shRNA with puro resistance and GFP markerDepositorInsertTAF4 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
MLSE-shRPA3.457
Plasmid#105584Purposeretrovirally express RPA3 shRNA with GFP markerDepositorInsertRPA3 shRNA #457
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[S1Apt]
Plasmid#68425PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct with S1 streptavidin aptamer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cdk12_intron3_sg2_pX330
Plasmid#127174PurposesgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backboneDepositorInsertMouse Cdk12 Intron 3 sgRNA
UseCRISPR and Mouse TargetingTagsExpressionMutationPromoterU6 PromoterAvailable SinceAvailabilityAcademic Institutions and Nonprofits only