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Plasmid#181721PurposeGalactose inducible expression of TDP-43-M337VDepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationM337VPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-TDP-43-N267S
Plasmid#181720PurposeGalactose inducible expression of TDP-43-N267SDepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationN267SPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-TDP-43-Q331K
Plasmid#181719PurposeGalactose inducible expression of TDP-43-Q331KDepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationQ331KPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS-R521C
Plasmid#181718PurposeGalactose inducible expression of FUS-R521CDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationR521CPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS-P431L
Plasmid#181717PurposeGalactose inducible expression of FUS-P431LDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationP431LPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector
Plasmid#194726PurposeCAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vectorDepositorArticleInsertAsCpf1(D908A)
UseCRISPRTagsExpressionMammalianMutationD908APromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX458-MAFB-sg
Plasmid#194720Purposepx458 with guide RNA that target hMAFBDepositorArticleInsertCas9 (MAFB Streptococcus pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
in-frame kanamycin backbone
Plasmid#177149PurposeExpresses kanamycin when ligated in-frameDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
in-frame ampicillin PKAKTR backbone
Plasmid#177197PurposePKAKTR mutagenesis template with in-frame ampicillin siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT074
Plasmid#180727PurposeSpacer cassette with ConL9 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert9/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT073
Plasmid#180726PurposeSpacer cassette with ConL8 and ConR9 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert8/9 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT072
Plasmid#180725PurposeSpacer cassette with ConL8 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert8/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT071
Plasmid#180724PurposeSpacer cassette with ConL7 and ConR8 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert7/8 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT070
Plasmid#180723PurposeSpacer cassette with ConL7 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert7/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT069
Plasmid#180722PurposeSpacer cassette with ConL6 and ConR7 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert6/7 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT068
Plasmid#180721PurposeSpacer cassette with ConL6 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert6/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT067
Plasmid#180720PurposeSpacer cassette with ConL5 and ConR6 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert5/6 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT066
Plasmid#180719PurposeSpacer cassette with ConL5 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert5/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT065
Plasmid#180718PurposeSpacer cassette with ConL4 and ConR5 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert4/5 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT064
Plasmid#180717PurposeSpacer cassette with ConL4 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert4/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT063
Plasmid#180716PurposeSpacer cassette with ConL3 and ConR4 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert3/4 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT062
Plasmid#180715PurposeSpacer cassette with ConL3 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert3/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT061
Plasmid#180714PurposeSpacer cassette with ConL2 and ConR3 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert2/3 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT060
Plasmid#180713PurposeSpacer cassette with ConL2 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert2/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT059
Plasmid#180712PurposeSpacer cassette with ConL1 and ConR2 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert1/2 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT058
Plasmid#180711PurposeSpacer cassette with ConL1 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsert1/E Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT057
Plasmid#180710PurposeSpacer cassette with ConLS and ConR1 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertS/1 Spacer Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVD1 tRNA-gRNA position E2 (GB2239)
Plasmid#160561PurposetRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNADepositorInserttRNA-gRNA position E2 (Multiplexing Edit)
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR78
Plasmid#90210PurposeExpresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyADepositorInsertsCcaS
Cph8
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
phU6-gRNA
Plasmid#53188PurposeExpresses the S. pyogenes sgRNA from the human U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pED17x11
Plasmid#134814PurposesfGFP expression plasmid for characterizing qtRNA chargingDepositorInsertsfGFP-151-TAGA
UseSynthetic BiologyTags6xHisExpressionBacterialMutation151-TAGAPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmU6-gRNA
Plasmid#53187PurposeExpresses the S. pyogenes sgRNA from the mouse U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromotermouse U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
ph7SK-gRNA
Plasmid#53189PurposeExpresses the S. pyogenes sgRNA from the 7SK promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman 7SKAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
phH1-gRNA
Plasmid#53186PurposeExpresses the S. pyogenes sgRNA from the H1 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman H1Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)
Plasmid#225756PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOS005: superecliptic pHluorin (cytosolic)
Plasmid#163049Purposesuperecliptic pHluorin (cytosolic)DepositorInsertsuperecliptic pHluorin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-nCas9n
Plasmid#47929Purposeexpression of an optimized Cas9 for genome-editing in zebrafishDepositorInsertnls-zcas9-nls
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-4
Plasmid#134802PurposeIPTG inducible expression of S-TAGA-Evo3 qtRNADepositorInsertS-TAGA-Evo3 qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDuex2-Dronpa
Plasmid#205756PurposeExpression of Dronpa in mamalian cellsDepositorInsertDronpa
UseTagsExpressionMammalianMutationwtPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29-neoLux1.2
Plasmid#229952PurposeBacterial expression for de novo luciferase, neoLux1.2DepositorInsertneoLux1.2
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP
Plasmid#165830PurposeEGFP green fluorescent protein CDSDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_HaloTag7_T2A_EGFP
Plasmid#169325PurposeExpression of HaloTag7 in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS011: superecliptic pHluorin (mitochondrial)
Plasmid#163050Purposesuperecliptic pHluorin (mitochondrial)DepositorInsertsuperecliptic pHluorin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV GG hUbC-dsRED
Plasmid#84034PurposeContains Golden Gate cassette to express up to four gRNAs and dsREDDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9
Plasmid#75381PurposedCas9DepositorInsertSp dCas9
UseLentiviralTagsnoExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDuex2-rsGreenF-E
Plasmid#205752PurposeExpression of rsGreenF-E in mamalian cellsDepositorInsertrsGreenF-E
UseTagsExpressionMammalianMutationwtPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mRuby4(1-10)
Plasmid#165143PurposeExpresses mRuby4(1-10) in mammalian cellsDepositorInsertmRuby4(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDuex2-Skylan62A
Plasmid#205755PurposeExpression of Skylan62A in mamalian cellsDepositorInsertSkylan62A
UseTagsExpressionMammalianMutationwtPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EBFP2(1-10)
Plasmid#165141PurposeExpresses EBFP2(1-10) in mammalian cellsDepositorInsertEBFP2(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits