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Ca-LiPPI-CLAPon1.0 (APEX)

Plasmid

#213408

Purpose

Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 1)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

MK2-E5-truncated hlov-TEVcs-APEX(201-250)-K5-HA-HA-APEX(2-200)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E4_47-300 (pBS0847)

Plasmid

#185279

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E4_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pXPA-fapO-RgTALsyn

Plasmid

#100946

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

GAP (Constitutive)

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1 E63A

Plasmid

#112861

Purpose

plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Use

Tags

Expression

Mammalian

Mutation

changed Glutamic acid 63 to Alanine

Promoter

CMV promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1-EGFP

Plasmid

#112857

Purpose

plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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ApoE2_mCh-SspB (pBS1142)

Plasmid

#185324

Purpose

For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck-ApoE3'UTR

Plasmid

#41846

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

ApoE 3'UTR (APOE Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
pDONR_P4-P1R_5'arm_apobb.1

Plasmid

#128662

Purpose

apobb.1 homology arm 5' gateway clone

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

Genomic sequence upstream of apobb.1 stop codon (apobb.1 Zebrafish)

Use

Multi-step gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR_P2R-P3_3'arm_apobb.1

Plasmid

#128681

Purpose

apobb.1 homology arm 3' gateway clone

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

apobb.1 (apobb.1 Zebrafish)

Use

Multi-step gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-NAT

Plasmid

#221129

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-KAN

Plasmid

#221128

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-CFAPorig

Plasmid

#221092

Purpose

FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-CUP1pr-NFAPoptim

Plasmid

#221110

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-CUP1pr-CFAPoptim

Plasmid

#221111

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-NFAPoptim

Plasmid

#221088

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-CFAPoptim

Plasmid

#221089

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-NFAPorig

Plasmid

#221091

Purpose

FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-NFAPoptim

Plasmid

#221093

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-CFAPoptim

Plasmid

#221094

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-ADH1pr-NFAPoptim

Plasmid

#221096

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge