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pTriEx-mVenus-Zdk1-RhoA Q63L

Plasmid

#81046

Purpose

mammalian expression of Zdk1 tagged RhoA Q63L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
RhoA Q63L (RHOA Human)

Use

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-Zdk1-RhoA Q63L

Plasmid

#81058

Purpose

mammalian expression of Zdk1 tagged RhoA Q63L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
RhoA Q63L (RHOA Human)

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-RHOA-G14V-deltaCAAX

Plasmid

#176097

Purpose

Constitutively active, nucelar localized RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RHOA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

G14V

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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RhoA sensor (LSS-SGFP2-Scarlet-I)

Plasmid

#112947

Purpose

RhoA FRET biosensor

Depositor

Dorus Gadella

Insert

RhoA sensor

Use

Tags

LSS-SGFP2 and mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-FRB-RhoA/N12

Plasmid

#214298

Purpose

Express split-RhoA fragment fused with FRB and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-RhoA fragment fused with FRB (RHOA Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Promoter

Tac

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b-RhoA/13C-FKBP-MBP

Plasmid

#214299

Purpose

Express split-RhoA fragment fused with FKBP and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-RhoA fragment fused with FKBP (RHOA Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

RhoA-Q63L

Promoter

T7

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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TOMM20-mTurquoise-RhoA-G14V-deltaCaaX

Plasmid

#176123

Purpose

Constitutively active, mitochondrial localized RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

G14V

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE-BI GFP10-RHOA/RBD-GFP11

Plasmid

#182232

Purpose

Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Inserts

ras homolog family member A (RHOA Human)
rhotekin RHO binding domain (RBD)

Use

Lentiviral

Tags

GFP10 (split-GFP tripartite) and GFP11 (M4)

Expression

Mutation

Promoter

TRE bidirectional

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-AHA740D, E758K-2A-Cherry-RhoAQ63L in pmCherryN1

Plasmid

#187288

Purpose

Express pmCherry-IL2-AH A740D, E758K-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH A740D, E758K, RhoA Q63L

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

Plasmid

#12153

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Use

Retroviral

Tags

6XHis

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2006

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA

Plasmid

#165435

Purpose

Expresses ShadowY-Rhotekin(8-89) and Clover(T153M/F223R)-RhoA in mammalian cells to monitor Cdc42 activity with FLIM imaging.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA

Use

Tags

ShadowY, Clover(T153M/F223R)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA

Plasmid

#165437

Purpose

Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-RhoA in mammalian cells as a negative control for FLIM imaging.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA

Use

Tags

ShadowY, Clover(T153M/F223R)

Expression

Mammalian

Mutation

H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…

Promoter

CMV

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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CaRQ

Plasmid

#34558

Depositor

Kevin Truong

Article

Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

Insert

CaRQ (RHOA Human)

Use

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L in RhoA

Promoter

CMV, T7

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-mTagBFP2-MRTF-A

Plasmid

#182061

Purpose

Mammalian expression vector encoding myocardin-related transcription factor A tagged with mTagBFP2.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mTagBFP2-MRTF-A (MRTFA Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLyn-CaRQ

Plasmid

#34559

Depositor

Kevin Truong

Article

Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

Insert

pLyn-CaRQ (RHOA Human)

Use

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L in RhoA

Promoter

CMV, T7

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-mTagBFP2-MRTF-B

Plasmid

#182062

Purpose

Mammalian expression vector encoding myocardin-related transcription factor B tagged with mTagBFP2.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mTagBFP2-MRTF-B (Mrtfb Mouse)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-rGBD

Plasmid

#26732

Depositor

William Bement

Article

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Insert

rhotekin (rtkn2 Mouse)

Use

Reporter for rho

Tags

GFP

Expression

Mammalian

Mutation

RhoA binding domain of rhotekin

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-mCherry-p115

Plasmid

#181996

Purpose

Mammalian expression vector encoding p115 RhoGEF tagged with mCherry.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mCherry-p115 (ARHGEF1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rho1 DN

Plasmid

#52231

Purpose

Drosophila expression of YFP-Rho1 dominant negative mutant

Depositor

Matthew Scott

Insert

Rho1 (Rho1 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T19N; DN mutation

Promoter

GAL4

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-mCherry-p115(S240A)

Plasmid

#181997

Purpose

Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mCherry-p115(S240A) (ARHGEF1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Serine 240 changed to alanine in p115.

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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