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We narrowed to 1,000 results for: biotin

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Showing: 101 - 120 of 1000 results

  4. pSBbi-Pur/GDI1-splitTurboID

    Plasmid
    #198219
    Purpose
    Transposon vector (Addgene #60523) for expression of part of TurboID (up to E256) and GID1, for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    GID1
    Use
    Transposon
    Tags
    5’ part of TurboID, up to E256
    Expression
    Mammalian
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198219-plasmid-map-sequence-id-389399

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  6. pET21-Flavidin

    Plasmid
    #89881
    Purpose
    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling
    Depositor
    Mark Howarth
    Article
    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-
    Insert
    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    Tags
    6 glutamate tag (C terminal on insert)
    Expression
    Bacterial
    Mutation
    K80R, K121R, K132R, K134R
    Available Since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89881-plasmid-map-sequence-id-177281

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  7. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid
    #198217
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198217-plasmid-map-sequence-id-389406

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  10. pSBtet-Bla/mCardinal-TurboID-GAI

    Plasmid
    #198218
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    mCardinal
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198218-plasmid-map-sequence-id-389373

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  11. pSBtet-Bla/GFP

    Plasmid
    #198221
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of EGFP
    Depositor
    Eric Davis
    Insert
    EGFP
    Use
    Transposon
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198221-plasmid-map-sequence-id-389395

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  12. Dead Streptavidin-SpyCatcher

    Plasmid
    #59547
    Purpose
    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead-dloop-Streptavidin-SpyCatcher
    Tags
    N-terminally shortened SpyCatcher
    Expression
    Bacterial
    Mutation
    PolyAsp in L3/4 loop for ion-exchange
    Available Since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available Since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pSBbi_RP_3XNLS-MicroID2-FLAG

    Plasmid
    #188523
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Article
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Insert
    3XNLS-MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188523-plasmid-map-sequence-id-372834

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  16. pSBtet-Bla/PAG1-GFP

    Plasmid
    #198220
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of human PAG1 fused to EGFP
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198220-plasmid-map-sequence-id-389402

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  19. pSBbi_RP_2XMTS-MicroID2-FLAG

    Plasmid
    #188524
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Article
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Insert
    2XMTS-MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188524-plasmid-map-sequence-id-372835

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Showing: 101 - 120 of 1000 results