We narrowed to 687 results for: emd

1. pACYC-Skp-TF

   Plasmid

   #83921

   Purpose

   Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   Trigger Factor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. His-TEV-PRC1

   Plasmid

   #167539

   Purpose

   For expression of His6-TEV-PRC1 in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

   Insert

   PRC1 (PRC1 Human)
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-SUPREM

   Plasmid

   #205023

   Purpose

   Expressing SUPer Rna Ecogii Methyltransferase (SUPREM)

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   SUPer Rna Ecogii Methyltransferase
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET16better_H10_HRV3C_chitinase

   Plasmid

   #175091

   Purpose

   Expresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.

   Depositor

   Jay C Dunlap

   Article

   Bartholomai et al Fungal Genet Biol. 2021 Aug 20;156:10361

   Insert

   chitinase
   

   Use

   Tags

   10X His, HRV-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET22b RNase A

   Plasmid

   #58903

   Purpose

   Bacterial expression of bovine pancreatic ribonuclease A

   Depositor

   Ronald Raines

   Article

   delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

   Insert

   RNase A (RNASE1 Bovine)
   

   Use

   Tags

   pelB sequence

   Expression

   Bacterial

   Mutation

   See Depositor Comments below

   Promoter

   T7

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. His-TEV-EGFP-PRC1

   Plasmid

   #167540

   Purpose

   For expression of His6-TEV-EGFP-PRC1 in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

   Insert

   PRC1 (PRC1 Human)
   

   Use

   Tags

   EGFP and His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-11-LECT2-I40

   Plasmid

   #183032

   Purpose

   Expression of LECT2 I40 variant protein from T7 promoter

   Depositor

   Gareth Morgan

   Article

   Belonogov et al bioRxiv 2022.03.01.482540

   Insert

   LECT2 (LECT2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I40 variant

   Promoter

   T7

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-AncMEcoGII291

   Plasmid

   #205024

   Purpose

   Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 291
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-T4uvsW

   Plasmid

   #228201

   Purpose

   Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

   Depositor

   Stephen Benkovic

   Article

   Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

   Insert

   uvsW
   

   Use

   Tags

   hexahistidine and 10 amino acid linker

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-AncMEcoGII250

    Plasmid

    #205025

    Purpose

    Expressing engineered M.EcoGII with higher thermostability

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 250
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET30-T7-His-sfGFP

    Plasmid

    #180755

    Purpose

    pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GSK3beta/pET29b

    Plasmid

    #16315

    Depositor

    Peter Klein

    Article

    Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

    Insert

    GSK3beta (gsk3b.L Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pSems LifeAct-StayGold

    Plasmid

    #222947

    Purpose

    Expression of LifeAct tagged with StayGold in mammalian cells

    Depositor

    Jacob Piehler

    Article

    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

    Insert

    LifeAct
    

    Use

    Tags

    StayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. His-TEV-SNAP-PRC1

    Plasmid

    #167541

    Purpose

    For expression of His6-TEV-SNAP-PRC1 in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

    Insert

    PRC1 (PRC1 Human)
    

    Use

    Tags

    His6 tag and SNAP tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-SdrG_N2N3-HIS-ybbr

    Plasmid

    #101238

    Purpose

    SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    SdrG_N2N3
    

    Use

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET29b-EPN-01*

    Plasmid

    #80423

    Purpose

    For expression of EPN-01* in E. coli

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01*
    

    Use

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pET30-T7-sfGFP

    Plasmid

    #180757

    Purpose

    pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET23b-proMMP-7

    Plasmid

    #29524

    Depositor

    William Parks

    Article

    Ra et al J Biol Chem. 2009 Oct 9. 284(41):27924-3

    Insert

    proMMP-7 (MMP7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pTNS2

    Plasmid

    #87802

    Purpose

    For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

    Depositor

    Yu-Zhu Zhang

    Article

    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTS1

    Plasmid

    #87797

    Purpose

    For constructing SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.

    Depositor

    Yu-Zhu Zhang

    Article

    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge