We narrowed to 6,417 results for: nls

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pRhobin2-SEC61B-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239024

Purpose

Co-overexpression of Rhobin2-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

Rhobin2-SEC61B (SEC61B Synthetic, Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

Plasmid

#171587

Purpose

Bacterial expression of NLS-RfxCas13d-NLS 6His

Depositor

Neville Sanjana

Article

Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

Insert

MKIEE-NLS-RfxCas13d-NLS-HA-6His

Tags

HA-6HIS and MKIEE

Expression

Bacterial

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dMisCas13b-SNAPf-NLS-3xFlag

Plasmid

#191536

Purpose

Overexpression of NLS-dMisCas13b-SNAPf-NLS-3xFlag in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dMisCas13b-SNAPf-NLS-3xFlag

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSt1374-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

Plasmid

#112209

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3L, DNMT3A Human)

Use

CRISPR

Mutation

D10A,H840A,N863A,C710S

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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nCre-ST-NLS-P2A-NLS-pSC-cCre

Plasmid

#207638

Purpose

A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre

Expression

Mammalian

Mutation

Amber stop codon at SpyCatcher’s critical lysine

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-nCas9-NLS-P2A-EGFP (pRZ70)

Plasmid

#123616

Purpose

CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

NLS-nCas9-NLS-P2A-EGFP

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CMV

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

Plasmid

#121835

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

Plasmid

#69219

Purpose

Expresses wild type SpCas9 fused to Zif268 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

Zif268 (Egr1 Mouse)

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

Plasmid

#69227

Purpose

Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

Plasmid

#69225

Purpose

Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS2 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS

Plasmid

#121834

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

x-Cas9(3.7) (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

Plasmid

#69226

Purpose

Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS

Plasmid

#121828

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

x-dCas9(3.7) (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT3-NLS-3XHA-NLS

Plasmid

#69223

Purpose

Expresses R1335K mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1335 is changed to Lysine

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAT1089-(PB-4_EF1α-Blast_2A_rtTA3 [HA-NLS-dCas9-NLS])

Plasmid

#251108

Purpose

Expression of dCas9 component of Casilio-i system

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

dCas9

Tags

HA

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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CAG-NLS-mScarleti-NLS-IRES-Puromycin

Plasmid

#234890

Purpose

Expressed a nuclear mScarleti fluorophoro in mammalian cells

Depositor

Nancy Papalopulu

Article

Miller et al Dev Cell. 2025 Sep 22;60(18):2518-2532.e

Insert

mScarleti

Tags

NLS on N- and C- terminals

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTOMM20-Rhobin2-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239022

Purpose

Co-overexpression of TOMM20-Rhobin2 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

TOMM20-Rhobin2 (TOMM20 Synthetic, Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTOMM20-Rhobin9-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239023

Purpose

Co-overexpression of TOMM20-Rhobin9 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

TOMM20-Rhobin9 (TOMM20 Synthetic, Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRhobin9-SEC61B-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239025

Purpose

Co-overexpression of Rhobin9-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

Rhobin9-SEC61B (SEC61B Synthetic, Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

Plasmid

#169596

Purpose

Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

dead S.pyogenes Cas9

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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