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Plasmid#149379PurposemUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangsDepositorInserttetA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLBH546_Tet-Cas14b1Locus
Plasmid#112503PurposeExpresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14b1
UseCRISPRTagsExpressionBacterialMutationPromoterTetAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLBH547_Tet-Cas14b2Locus
Plasmid#112504PurposeExpresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14b2
UseCRISPRTagsExpressionBacterialMutationPromoterTetAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSECRETS-A
Plasmid#196986PurposeFor SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpltetO1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-cpLuc
Plasmid#135628PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V1)DepositorInsertNAD-cpLuc sensor
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
stathmin-CFP
Plasmid#86783PurposeMammalian expression of stathmin-CFPDepositorInsertstathmin (STMN1 Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-Luc
Plasmid#135627PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V0)DepositorInsertNAD-Luc sensor
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
hPRL3_1-169
Plasmid#209796PurposeBacterial expression of human PRL-3 without farnesylation motifDepositorInsertPRL-3 (PTP4A3 Human)
UseTags6xHisExpressionBacterialMutationaa 1-169 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Aahn
Plasmid#112158PurposeA genetically encoded voltage indicator (Aahn), capable of optically monitoring both plasma membrane and internal membrane potentials.DepositorInsertAahn
UseTagsSuper ecliptic pHlorin A227DExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pFA6a-GST-TRP1
Plasmid#41603DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTagsGST and yeast ADH1 terminatorExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVC-CRISPR
Plasmid#120369PurposePlasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing libraryDepositorInsertsCRISPR1
CRISPR2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
a5-ecto-pcDNA31hygro
Plasmid#113386PurposeExpress human alpha5 ectodomain with secretion peptide, purification tags and C-terminal claspDepositorInserthuman alpha 5 ectodomain
UseTagsStrep II tagExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FnR9-10
Plasmid#182567PurposeHuman fibronectin fragment, type-III repeats 9-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Elk1 pShuttle
Plasmid#121097PurposeExpression of full length ELK1 in mammalian cellsDepositorInsertELK1 (Elk1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
a4 ecto pcDNA3.1 hygro(-)
Plasmid#113406Purposeexpression of human alpha 4 ectodomain with purification tags and C-terminal claspDepositorInserthuman alpha 4 ectodomain
UseTagsStrep II tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1
Plasmid#41606DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsTRP1 reverse orientationExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
Integrin-β6 I domain I270C
Plasmid#159639PurposeContains integrin beta 6 residues 108–352 with I270C mutationDepositorInsertIntegrin beta 6 I domain I270C
UseTags6x HisExpressionMammalianMutationI270CPromoterAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pvTRAP
Plasmid#50047Purposeexpresses plasmodium vivax TRAP adhesion domains (25-283) in mammalian cellsDepositorInsertplasmodium vivax TRAP
UseTags6Xhis tagExpressionMammalianMutationS42Q, N91S, N128S, S180RPromotercmvAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXTK018 [Origin_pUC_PartType1]
Plasmid#229255PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_pUC_PartType1DepositorInsertpUC Origin
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only