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CMV-HA-hTAF6

Plasmid

#17988

Depositor

Naoko Tanese

Article

Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

Insert

TAF6 (TAF6 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
pICSL1P1_AtU626Ter26

Plasmid

#191769

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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CycA-Venus-Flag (1305)

Plasmid

#39853

Depositor

Jonathon Pines

Article

Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

Insert

Cyclin A (CCNA2 Human)

Tags

Flag and Venus

Expression

Mammalian

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
EC70199

Plasmid

#209460

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFUM

Plasmid

#133130

Purpose

A recombinant plasmid expressing fum (fumarase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

fumarase gene

Expression

Bacterial

Promoter

PEP-carboxykinase promoter

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-aequorin

Plasmid

#101010

Purpose

Construct for expression of aequorin, calcium sensitive luciferase reporter and hygromycin resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMT-Diaphanous-GFP

Plasmid

#24287

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Diaphanous (dia Fly)

Tags

GFP

Expression

Insect

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
P526

Plasmid

#139740

Purpose

Individual component of Caffeic Acid biosynthesis pathway, hpaC

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

hpaC

Expression

Plant

Promoter

35S

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pTH1-SpSrtA

Plasmid

#64987

Purpose

Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

S. pyogenes sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-81

Promoter

T7 promoter

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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MSCVhyg-F-Axin1

Plasmid

#24928

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Axin1 (AXIN1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

June 27, 2010

Availability

Academic Institutions and Nonprofits only
P525

Plasmid

#139739

Purpose

Individual component of Caffeic Acid biosynthesis pathway, hpaB

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

hpaB

Expression

Plant

Promoter

35S

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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P527

Plasmid

#139741

Purpose

Individual component of Caffeic Acid biosynthesis pathway, RcTAL

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

TAL

Expression

Plant

Promoter

35S

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSL690

Plasmid

#47753

Purpose

Expresses dCas9-VP64 fusion

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Tags

3x FLAG, NLS, and VP64

Expression

Mammalian

Mutation

D10A and H840A mutations in Cas9

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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EC70201

Plasmid

#209462

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET41-GST-KaiC

Plasmid

#68051

Purpose

Strain: AM5117, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiC

Tags

6xHis, GST, and S-Tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCO706

Plasmid

#139743

Purpose

Individual component of Caffeic Acid biosynthesis pathway, hpaC

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

hpaB

Use

Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCO707

Plasmid

#139744

Purpose

Individual component of Caffeic Acid biosynthesis pathway, hpaC

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

hpaC

Use

Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCO708

Plasmid

#139745

Purpose

Individual component of Caffeic Acid biosynthesis pathway, hpaC

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

TAL

Use

Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Six1 (CT#664)

Plasmid

#13876

Depositor

Cliff Tabin

Article

Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

Insert

Six1 (Six1 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pET28a-hdNadV

Plasmid

#83362

Purpose

Expresses nadV from Haemophilus ducreyi in Escherichia coli

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

Insert

nadV

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 10 mut B-Myb

Plasmid

#25966

Depositor

Rob Lewis

Article

Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

Insert

B-Myb (Mybl2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed threonines 267, 408, 497, 519, 522 and 52…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pET28-SUMO-KaiA

Plasmid

#68049

Purpose

Strain: AM5115, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiA

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-SUMO-KaiB

Plasmid

#68050

Purpose

Strain: AM5116, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiB

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR4_EWSR1

Plasmid

#26367

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

EWSR1 (EWSR1 Human)

Use

Entry vector

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pGEMT-T-HSV2.3 TCR alpha chain

Plasmid

#74076

Purpose

gBT-I TCR a chain cDNA in pGEM-T

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR alpha clone HSV2.3 (Tcra Mouse)

Expression

Bacterial

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSico Spry-2

Plasmid

#14809

Depositor

Tyler Jacks

Article

Shaw et al Genes Dev. 2007 Mar 15. 21(6):694-707.

Insert

Sprouty-2 shRNA (Spry2 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pWZLblast-RalA WT(RalA 402i-resistant)

Plasmid

#11124

Depositor

Christopher Counter

Article

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalA WT (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

resistant to siRNA 402i

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pICSL1P3_AtU626Ter26

Plasmid

#191771

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCKTRBS-OD

Plasmid

#102924

Purpose

pCK01 derivative carrying tetR under a constitutive promoter and an aTc-inducible PtetO promoter for control expression of genes. Contains LacI OD for translational fusions on the Ct of cloned genes.

Depositor

George Church

Article

Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

Type

Empty backbone

Expression

Bacterial

Promoter

PtetO

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pWZ414-F55

Plasmid

#23079

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R …

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only