We narrowed to 2,404 results for: hal.2

1. pGL3b(1454/3172)P2P-wt

   Plasmid

   #21404

   Depositor

   Eric Metzen

   Article

   Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

   Insert

   EGLN1 (EGLN1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

2. CRY2-mCherry-Miro1TM

   Plasmid

   #102247

   Purpose

   Expresses fusion of CRY2PHR with mCherry and transmembrane domain of Miro1

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   CRY2PHR-mCherry-Miro1TM (RHOT1, CRY2 Mustard Weed, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRN120

   Plasmid

   #160696

   Purpose

   BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Inserts

   CmYLCV::Luc+::AtHSP
   Nos::WUS2::PinII
   AtUbi10::STM::35S
   

   Use

   Luciferase; Gemini viral replicon, plant developm…

   Promoter

   AtUbi10, CmYLCV, and Nos

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSH-EFIRES-P-AtAFB2-mCherry-weak NLS

   Plasmid

   #129717

   Purpose

   AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLS

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry-weak NLS

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF1a

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV pCAG-mRuby3-WPRE

   Plasmid

   #107744

   Purpose

   Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

   Depositor

   Rylan Larsen

   Article

   Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

   Insert

   mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Zebrafish aBb crystallin 2kb promoter/AcGFP

   Plasmid

   #98099

   Purpose

   Zebrafish aBb crystallin 2kb promoter segment driving GFP expression

   Depositor

   Mason Posner

   Article

   Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

   Insert

   Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)
   

   Use

   Gfp expressing

   Tags

   GFP

   Promoter

   Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-dCas9-T2A-GFP

   Plasmid

   #193781

   Purpose

   Tricistronic expression of dCas9-EGFP-BSD

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   dead Cas9
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   In frame with existing Cas9 promoter

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRE-BI-osTIR1

   Plasmid

   #207840

   Purpose

   Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

   Depositor

   Michaela Mueller-McNicoll

   Article

   Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

   Inserts

   osTIR
   mCherry
   mAID_-VHH-GFP4
   

   Tags

   3X HA-Tag and weak NLS

   Expression

   Mammalian

   Mutation

   All K mutated to R

   Promoter

   TRE3G BI promoter

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CIB-VP64 (B1016)

   Plasmid

   #92037

   Purpose

   Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CIB1-VP64 (CIB1 Mustard Weed)
   

   Tags

   Fusion of plant CIB1 with VP64 activation domain.…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

    Plasmid

    #182395

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-VenusN154
    VNp-LZ-VenusC155
    

    Tags

    Vesicle Nucleating peptide (VNp) & Leucine Zi…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCas9-T2A-mApple

    Plasmid

    #193780

    Purpose

    Tricistronic expression of Cas9-mApple-BSD in mammalian cells

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    mApple
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFRT-DHX36iso2E335A

    Plasmid

    #159588

    Purpose

    Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

    Depositor

    Markus Hafner

    Article

    Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

    Insert

    DHX36 (DHX36 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    E335A

    Promoter

    CMV

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-FKBP-IRSp53(trunc C2)

    Plasmid

    #221274

    Purpose

    Expression of IRSp53 C-terminal truncation 2 (247-335) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    IRSp53 C-terminal truncation 2 (247-335) (Baiap2 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiGuide-puro_Panc480-MT7

    Plasmid

    #200941

    Purpose

    Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    multiplexed sgRNA array
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, EF-1a

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PHR-GFP-VP16

    Plasmid

    #183927

    Purpose

    Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP-VP16 (CRY2, UL48 Mustard Weed, Synthetic)
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. LAMP-mCherry-CRY2

    Plasmid

    #102249

    Purpose

    Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    LAMP-mCherry-CRY2 (LAMP1, CRY2 Mustard Weed, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PHR-GFP-FUSN-VP16

    Plasmid

    #183933

    Purpose

    Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP-FUSN-VP16 (CRY2, UL48 Mustard Weed, Synthetic)
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB3omega1_KanR_BastaR

    Plasmid

    #186426

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PEX-mCherry-CRY2

    Plasmid

    #102250

    Purpose

    Expresses fusion of CRY2PHR with mCherry and PEX3 (1-42)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    PEX-mCherry-CRY2 (PEX3, CRY2 Mustard Weed, Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-CRY2-P14P5K

    Plasmid

    #79570

    Purpose

    expression of mCherry-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c

    Depositor

    Olof Idevall-Hagren

    Article

    Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26

    Insert

    Pip5k1c (Pip5k1c Mouse)
    

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    R445E and R446E; truncation of amino acids 636-66…

    Promoter

    CMV

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only