We narrowed to 2,542 results for: pcas9

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pEPOR2KN0010

Plasmid

#117582

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0009

Plasmid

#117581

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0008

Plasmid

#117580

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0004

Plasmid

#117579

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0023)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0003

Plasmid

#117578

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0022)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0001

Plasmid

#117576

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0114)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0015

Plasmid

#117525

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (no stop codon)

Use

CRISPR

Mutation

K855A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0001

Plasmid

#117523

Purpose

Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-DE (no stop codon)

Use

CRISPR

Mutation

D1135E

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0049

Plasmid

#117616

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pX330-Cetn1/1

Plasmid

#50718

Purpose

pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Inserts

Cetn1 sgRNA1
humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh and hU6

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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p101_CRISPRai_PiggyBac

Plasmid

#213777

Purpose

Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

VPR-dSaCas9
dSpCas9-KRAB-BFP
zeocin
Tet-On transactivator

Use

CRISPR

Promoter

EF1a (EF-1-alpha intron a) and Tet_On_TRE

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-EN1201

Plasmid

#92144

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pZS157 CRISPEY RT/Cas9

Plasmid

#114454

Purpose

Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Insert

SpCas9, Ec86-RT

Use

CRISPR

Tags

SV40-NLS

Expression

Yeast

Mutation

S. cerevisiae codon optimized

Promoter

GAL1-GAL10

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAID6

Plasmid

#136989

Purpose

Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Inserts

dLbCpf1-VP
dSpCas9-RD1152
SaCas9
Csy4

Expression

Yeast

Promoter

ENO2, TDH3, TEF1, and TPI1

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-BsaIx2-DD-Cas9

Plasmid

#75380

Purpose

Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

DD-SpCas9

Use

CRISPR

Tags

FKBP12 L106P DD

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBECKP-spe

Plasmid

#117236

Purpose

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-Cas9

Plasmid

#89995

Purpose

Expresses high levels of WT Cas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

Insert

hCas9

Tags

3xFlag, SV40 NLS, and T2A peptide

Expression

Mammalian

Promoter

CAG

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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SiC-V1-Scr

Plasmid

#133042

Purpose

SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

dTomato

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGEL063

Plasmid

#124895

Purpose

For genome editing in plant by SpCas9 variant(SpCas9-NG)

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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